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Chemical synthesis of circular proteins.

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Chemical synthesis of circular proteins.
นักวิจัย : Tam, James P. , Wong, Clarence T. T.
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Tam, J. P., & Wong, C. T. T. (2012). Chemical synthesis of circular proteins. Journal of biological chemistry, 287(32), 27020-27025. , http://hdl.handle.net/10220/17091 , http://dx.doi.org/10.1074/jbc.R111.323568
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of biological chemistry
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Circular proteins, once thought to be rare, are now commonly found in plants. Their chemical synthesis, once thought to be difficult, is now readily achievable. The enabling methodology is largely due to the advances in entropic chemical ligation to overcome the entropy barrier in coupling the N- and C-terminal ends of large peptide segments for either intermolecular ligation or intramolecular ligation in end-to-end cyclization. Key elements of an entropic chemical ligation consist of a chemoselective capture step merging the N and C termini as a covalently linked O/S-ester intermediate to permit the subsequent step of an intramolecular O/S-N acyl shift to form an amide. Many ligation methods exploit the supernucleophilicity of a thiol side chain at the N terminus for the capture reaction, which makes cysteine-rich peptides ideal candidates for the entropy-driven macrocyclization. Advances in desulfurization and modification of the thiol-containing amino acids at the ligation sites to other amino acids add extra dimensions to the entropy-driven ligation methods. This minireview describes recent advances of entropy-driven ligation to prepare circular proteins with or without a cysteinyl side chain.

บรรณานุกรม :
Tam, James P. , Wong, Clarence T. T. . (2555). Chemical synthesis of circular proteins..
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Tam, James P. , Wong, Clarence T. T. . 2555. "Chemical synthesis of circular proteins.".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Tam, James P. , Wong, Clarence T. T. . "Chemical synthesis of circular proteins.."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Tam, James P. , Wong, Clarence T. T. . Chemical synthesis of circular proteins.. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.