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Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A1AO ATP synthase of Methanosarcina mazei Gö1

หน่วยงาน Nanyang Technological University, Singapore

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ชื่อเรื่อง : Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A1AO ATP synthase of Methanosarcina mazei Gö1
นักวิจัย : Raghunathan, Devanathan , Gayen, Shovanlal , Kumar, Anil , Hunke, Cornelia , Grüber, Gerhard , Verma, Chandra S.
คำค้น : DRNTU::Science::Biological sciences
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Raghunathan, D., Gayen, S., Kumar, A., Hunke, C., Grüber, G., & Verma, C. S. (2012). Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A1AO ATP synthase of Methanosarcina mazei Gö1. Journal of bioenergetics and biomembranes, 44(1), 213-224. , 0145-479X , http://hdl.handle.net/10220/17008 , http://dx.doi.org/10.1007/s10863-012-9410-y
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of bioenergetics and biomembranes
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The interaction of the nucleotide-binding subunit B with subunit F is essential in coupling of ion pumping and ATP synthesis in A1AO ATP synthases. Here we provide structural and thermodynamic insights on the nucleotide binding to the surface of subunits B and F of Methanosarcina mazei Gö1 A1AO ATP synthase, which initiated migration to its final binding pocket via two transitional intermediates on the surface of subunit B. NMR- and fluorescence spectroscopy as well as ITC data combined with molecular dynamics simulations of the nucleotide bound subunit B and nucleotide bound B-F complex in explicit solvent, suggests that subunit F is critical for the migration to and eventual occupancy of the final binding site by the nucleotide of subunit B. Rotation of the C-terminus and conformational changes in subunit B are initiated upon binding with subunit F causing a perturbation that leads to the migration of ATP from the transition site 1 through an intermediate transition site 2 to the final binding site 3. This mechanism is elucidated on the basis of change in binding affinity for the nucleotide at the specific sites on subunit B upon complexation with subunit F. The change in enthalpy is further explained based on the fluctuating local environment around the binding sites.

บรรณานุกรม :
Raghunathan, Devanathan , Gayen, Shovanlal , Kumar, Anil , Hunke, Cornelia , Grüber, Gerhard , Verma, Chandra S. . (2555). Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A1AO ATP synthase of Methanosarcina mazei Gö1.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Raghunathan, Devanathan , Gayen, Shovanlal , Kumar, Anil , Hunke, Cornelia , Grüber, Gerhard , Verma, Chandra S. . 2555. "Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A1AO ATP synthase of Methanosarcina mazei Gö1".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Raghunathan, Devanathan , Gayen, Shovanlal , Kumar, Anil , Hunke, Cornelia , Grüber, Gerhard , Verma, Chandra S. . "Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A1AO ATP synthase of Methanosarcina mazei Gö1."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Raghunathan, Devanathan , Gayen, Shovanlal , Kumar, Anil , Hunke, Cornelia , Grüber, Gerhard , Verma, Chandra S. . Subunit F modulates ATP binding and migration in the nucleotide-binding subunit B of the A1AO ATP synthase of Methanosarcina mazei Gö1. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.