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Kainate receptors mediate regulated exocytosis of secretory phospholipase A2 in SH-SY5Y neuroblastoma cells

หน่วยงาน Nanyang Technological University, Singapore

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ชื่อเรื่อง : Kainate receptors mediate regulated exocytosis of secretory phospholipase A2 in SH-SY5Y neuroblastoma cells
นักวิจัย : Than, Aung , Tan, Yan , Ong, Wei-Yi , Farooqui, Akhlaq A. , Chen, Peng
คำค้น : DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2554
อ้างอิง : Than, A., Tan, Y., Ong, W. Y., Farooqui, A. A., & Chen P. (2012). Kainate Receptors Mediate Regulated Exocytosis of Secretory Phospholipase A2 in SH-SY5Y Neuroblastoma Cells. Neurosignals, 20(2), 72-85. , http://hdl.handle.net/10220/16680 , http://dx.doi.org/10.1159/000330414
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Neurosignals
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Secretory phospholipase A(2) (sPLA(2)) isoforms are widely expressed in the brain and spinal cord. Group IIA sPLA(2) (sPLA(2)-IIA) has been shown to stimulate exocytosis and release of neurotransmitters in neuroendocrine PC12 cells and neurons, suggesting a role of the enzyme in neuronal signaling and synaptic transmission. However, the mechanisms by which sPLA(2) is itself released, and a possible relation between glutamate receptors and sPLA(2) exocytosis, are unknown. This study was carried out to elucidate the effects of glutamate receptor agonists on exocytosis of sPLA(2)-IIA in transfected SH-SY5Y neuroblastoma cells. sPLA(2)-IIA enzyme was packaged in fusion-competent vesicles and released constitutively or upon stimulation, suggesting regulated secretion. The signal peptide of sPLA(2)-IIA is required for its vesicular localization and exocytosis. External application of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and kainate (KA) induced vesicular exocytosis and release of sPLA(2)-IIA. UBP 302, a GluR5-specific KA receptor antagonist, abolished the effect of KA, confirming the role of KA receptors in mediating sPLA(2)-IIA secretion. Moreover, KA-induced sPLA(2)-IIA secretion is dependent on Ca(2+) and protein kinase C. Together, these findings provide evidence of a link between glutamate receptors and regulated sPLA(2) secretion in neurons that may play an important role in synaptic plasticity, pain transmission and neurodegenerative diseases.

บรรณานุกรม :
Than, Aung , Tan, Yan , Ong, Wei-Yi , Farooqui, Akhlaq A. , Chen, Peng . (2554). Kainate receptors mediate regulated exocytosis of secretory phospholipase A2 in SH-SY5Y neuroblastoma cells.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Than, Aung , Tan, Yan , Ong, Wei-Yi , Farooqui, Akhlaq A. , Chen, Peng . 2554. "Kainate receptors mediate regulated exocytosis of secretory phospholipase A2 in SH-SY5Y neuroblastoma cells".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Than, Aung , Tan, Yan , Ong, Wei-Yi , Farooqui, Akhlaq A. , Chen, Peng . "Kainate receptors mediate regulated exocytosis of secretory phospholipase A2 in SH-SY5Y neuroblastoma cells."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2554. Print.
Than, Aung , Tan, Yan , Ong, Wei-Yi , Farooqui, Akhlaq A. , Chen, Peng . Kainate receptors mediate regulated exocytosis of secretory phospholipase A2 in SH-SY5Y neuroblastoma cells. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2554.