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Combination of microscopic techniques reveals a comprehensive visual impression of biofilm structure and composition

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Combination of microscopic techniques reveals a comprehensive visual impression of biofilm structure and composition
นักวิจัย : Alhede, Morten , Qvortrup, Klaus , Liebrechts, Ramon , Høiby, Niels , Givskov, Michael , Bjarnsholt, Thomas
คำค้น : DRNTU::Engineering::Bioengineering
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Alhede, M., Qvortrup, K., Liebrechts, R., Høiby, N., Givskov, M., & Bjarnsholt, T. (2012). Combination of microscopic techniques reveals a comprehensive visual impression of biofilm structure and composition. FEMS immunology & medical microbiology, 65(2), 335-342. , http://hdl.handle.net/10220/16252 , http://dx.doi.org/10.1111/j.1574-695X.2012.00956.x
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : FEMS immunology & medical microbiology
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Bacterial biofilms are imaged by various kinds of microscopy including confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). One limitation of CLSM is its restricted magnification, which is resolved by the use of SEM that provides high-magnification spatial images of how the single bacteria are located and interact within the biofilm. However, conventional SEM is limited by the requirement of dehydration of the samples during preparation. As biofilms consist mainly of water, the specimen dehydration might alter its morphology. High magnification yet authentic images are important to understand the physiology of biofilms. We compared conventional SEM, Focused Ion Beam (FIB)-SEM and CLSM with SEM techniques [cryo-SEM and environmental-SEM (ESEM)] that do not require dehydration. In the case of cryo-SEM, the biofilm is not dehydrated but kept frozen to obtain high-magnification images closer to the native state of the sample. Using the ESEM technique, no preparation is needed. Applying these methods to biofilms of Pseudomonas aeruginosa showed us that the dehydration of biofilms substantially influences its appearance and that a more authentic biofilm image emerges when combining all methods.

บรรณานุกรม :
Alhede, Morten , Qvortrup, Klaus , Liebrechts, Ramon , Høiby, Niels , Givskov, Michael , Bjarnsholt, Thomas . (2555). Combination of microscopic techniques reveals a comprehensive visual impression of biofilm structure and composition.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Alhede, Morten , Qvortrup, Klaus , Liebrechts, Ramon , Høiby, Niels , Givskov, Michael , Bjarnsholt, Thomas . 2555. "Combination of microscopic techniques reveals a comprehensive visual impression of biofilm structure and composition".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Alhede, Morten , Qvortrup, Klaus , Liebrechts, Ramon , Høiby, Niels , Givskov, Michael , Bjarnsholt, Thomas . "Combination of microscopic techniques reveals a comprehensive visual impression of biofilm structure and composition."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Alhede, Morten , Qvortrup, Klaus , Liebrechts, Ramon , Høiby, Niels , Givskov, Michael , Bjarnsholt, Thomas . Combination of microscopic techniques reveals a comprehensive visual impression of biofilm structure and composition. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.