ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization
นักวิจัย : Adav, Sunil S. , Ravindran, Anita , Cheow, Esther Sok Hwee , Sze, Siu Kwan
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Adav, S. S., Ravindran, A., Cheow, E. S. H., & Sze, S. K. (2012). Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization. Journal of Proteomics, 75(18), 5590–5603. , http://hdl.handle.net/10220/13688 , http://dx.doi.org/10.1016/j.jprot.2012.08.011
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of proteomics
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Proteomics analysis of lignocellulolytic proteins by lignocellulosic biomass degrading microbes and compatible microbial consortium is a promising approach that offers a new means to enzyme discovery. The abundance of proteins in complex secretome by microbial communities would highlight key lignocellulolytic proteins for lignocellulosic biorefinery. In this study, lignocellulolytic enzymes of potent lignin degrading basidiomycota and effective cellulolytic ascomycota fungal strains, and their co-cultures were analyzed using high throughput isobaric tag for relative and absolute quantitation (iTRAQ) technique using liquid chromatography tandem mass spectrometry. Protein abundances in the iTRAQ-multiplexed samples were determined by integrating relative quantitation and exponentially modified protein abundance index (emPAI). The functional classification of the secretory proteins by individual culture and co-culture demonstrated 36.77% cellulolytic proteins, 13.06% hemicellulases, 14.09% ligninolytic proteins, 19.59% proteolytic enzymes. 7.22% hypothetical proteins and 6.87% cell morphogenesis proteins. The abundance of the proteins by individual cultures and co-cultured fungal consortium revealed that co-culturing of Phanerochaete chrysosporium with Trichoderma reesei QM6a and Trichoderma reesei Rut C30 induced the production of cellulolytic proteins and stimulated expression of hemicellulolytic enzymes. The hierarchical clustering of proteins in secretome of fungal strains and their co-cultures elucidated differential expressions of lignocellulolytic proteins by the microbial consortium.

บรรณานุกรม :
Adav, Sunil S. , Ravindran, Anita , Cheow, Esther Sok Hwee , Sze, Siu Kwan . (2555). Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Adav, Sunil S. , Ravindran, Anita , Cheow, Esther Sok Hwee , Sze, Siu Kwan . 2555. "Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Adav, Sunil S. , Ravindran, Anita , Cheow, Esther Sok Hwee , Sze, Siu Kwan . "Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Adav, Sunil S. , Ravindran, Anita , Cheow, Esther Sok Hwee , Sze, Siu Kwan . Quantitative proteomic analysis of secretome of microbial consortium during saw dust utilization. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.