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Human keratin hydrogels support fibroblast attachment and proliferation in vitro

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Human keratin hydrogels support fibroblast attachment and proliferation in vitro
นักวิจัย : Wang, Shuai , Taraballi, Francesca , Tan, Lay Poh , Ng, Kee Woei
คำค้น : DRNTU::Engineering::Materials::Biomaterials.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Wang, S., Taraballi, F., Tan, L. P.,& Ng, K. W. (2012). Human keratin hydrogels support fibroblast attachment and proliferation in vitro. Cell and Tissue Research, 347(3), 795-802. , http://hdl.handle.net/10220/12798 , http://dx.doi.org/10.1007/s00441-011-1295-2
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Cell and tissue research
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Human hair keratins have a strong potential for development as clinically relevant biomaterials because they are abundant and bioactive and are a realistic source of autologous proteins. Specifically, keratins have the propensity to polymerize in an aqueous environment to form hydrogels. In order to evaluate the suitability of keratin hydrogels as substrates for cell culture, we have fabricated hydrogels using keratins extracted from human hair by inducing polymerization with Ca2+; these hydrogels exhibit highly branched and porous micro-architectures. L929 murine fibroblasts have been used in a preliminary cell culture study to compare the in vitro biocompatibility of the keratin hydrogels with collagen type 1 hydrogels of similar viscoelastic properties. Our results reveal that keratin hydrogels are comparable with collagen hydrogels in terms of the promotion of cell adhesion, proliferation and the preservation of cell viability. Interestingly, cells remain clustered in proliferative colonies within the keratin hydrogels but are homogeneously distributed as single cells in collagen hydrogels. Collectively, our results demonstrate that keratin hydrogels can be used as substrates for cell culture. Such gels might find applications as templates for soft tissue regeneration.

บรรณานุกรม :
Wang, Shuai , Taraballi, Francesca , Tan, Lay Poh , Ng, Kee Woei . (2555). Human keratin hydrogels support fibroblast attachment and proliferation in vitro.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Wang, Shuai , Taraballi, Francesca , Tan, Lay Poh , Ng, Kee Woei . 2555. "Human keratin hydrogels support fibroblast attachment and proliferation in vitro".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Wang, Shuai , Taraballi, Francesca , Tan, Lay Poh , Ng, Kee Woei . "Human keratin hydrogels support fibroblast attachment and proliferation in vitro."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Wang, Shuai , Taraballi, Francesca , Tan, Lay Poh , Ng, Kee Woei . Human keratin hydrogels support fibroblast attachment and proliferation in vitro. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.