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Identification and evaluation of apoptotic compounds from Garcinia Oligantha

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Identification and evaluation of apoptotic compounds from Garcinia Oligantha
นักวิจัย : Gao, Xue-Mei , Yu, Ting , Cui, Ming-Zhu , Pu, Jian-Xin , Du, Xue , Han, Quan-bin , Hu, Qiu-Fen , Liu, Tian-Cheng , Luo, Kathy Qian , Xu, Hong-Xi
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Gao, X. M., Yu, T., Cui, M. z., Pu, J. X., Du, X., Han, Q. b., Hu, Q. f., Liu, T. C., Luo, K. Q.,& Xu, H. X. (2012). Identification and evaluation of apoptotic compounds from Garcinia oligantha. Bioorganic & Medicinal Chemistry Letters, 22(6), 2350-2353. , 0960-894X , http://hdl.handle.net/10220/12781 , http://dx.doi.org/10.1016/j.bmcl.2012.01.068
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Bioorganic & medicinal chemistry letters
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Four new compounds, oliganthins A–D (1–4), and one known caged xanthone gaudichaudione H (5) were isolated from the stems of Garcinia oligantha. The structures of the new compounds were elucidated by spectroscopic evidences. All of the five compounds were evaluated for their apoptosis-inducing effects using HeLa-C3 cells which have been genetically engineered to produce a fluorescent biosensor capable of detecting caspase-3 activation. All of them induced cell apoptosis at 10 μM or lower concentrations. The apoptotic activity of oliganthins A, B and gaudichaudione H were further confirmed by detecting the cleavage of PARP, which is the substrate of activated caspase-3, in these compounds-treated cells using the method of Western blot. Moreover, the values of IC50 were measured for all five compounds on HeLa cells using the MTT assay. Among them, gaudichaudione H had the lowest IC50 value of 0.90 μM, while the other four new compounds had IC50 values of 1.58, 1.52, 4.15, and 7.82 μM, respectively. These results show that gaudichaudione H has the strongest apoptosis-inducing effect and cell growth inhibition effect among these xanthones and it may have the potential to be developed into a new anticancer agent.

บรรณานุกรม :
Gao, Xue-Mei , Yu, Ting , Cui, Ming-Zhu , Pu, Jian-Xin , Du, Xue , Han, Quan-bin , Hu, Qiu-Fen , Liu, Tian-Cheng , Luo, Kathy Qian , Xu, Hong-Xi . (2555). Identification and evaluation of apoptotic compounds from Garcinia Oligantha.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Gao, Xue-Mei , Yu, Ting , Cui, Ming-Zhu , Pu, Jian-Xin , Du, Xue , Han, Quan-bin , Hu, Qiu-Fen , Liu, Tian-Cheng , Luo, Kathy Qian , Xu, Hong-Xi . 2555. "Identification and evaluation of apoptotic compounds from Garcinia Oligantha".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Gao, Xue-Mei , Yu, Ting , Cui, Ming-Zhu , Pu, Jian-Xin , Du, Xue , Han, Quan-bin , Hu, Qiu-Fen , Liu, Tian-Cheng , Luo, Kathy Qian , Xu, Hong-Xi . "Identification and evaluation of apoptotic compounds from Garcinia Oligantha."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Gao, Xue-Mei , Yu, Ting , Cui, Ming-Zhu , Pu, Jian-Xin , Du, Xue , Han, Quan-bin , Hu, Qiu-Fen , Liu, Tian-Cheng , Luo, Kathy Qian , Xu, Hong-Xi . Identification and evaluation of apoptotic compounds from Garcinia Oligantha. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.