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Synthesis and cell adhesive properties of linear and cyclic RGD functionalized polynorbornene thin films.

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Synthesis and cell adhesive properties of linear and cyclic RGD functionalized polynorbornene thin films.
นักวิจัย : Patel, Paresma R. , Kiser, Rosemary Conrad. , Lu, Ying Y. , Fong, Eileen. , Ho, Wilson C. , Tirrell, David A. , Grubbs, Robert H.
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Patel, P. R., Kiser, R. C., Lu, Y. Y., Fong, E., Ho, W. C., Tirrell, D. A.,& Grubbs, R. H. (2012). Synthesis and Cell Adhesive Properties of Linear and Cyclic RGD Functionalized Polynorbornene Thin Films. Biomacromolecules, 13(8), 2546-2553. , http://hdl.handle.net/10220/12839 , http://dx.doi.org/10.1021/bm300795y
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Biomacromolecules
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Described herein is the efficient synthesis and evaluation of bioactive arginine-glycine-aspartic acid (RGD) functionalized polynorbornene-based materials for cell adhesion and spreading. Polynorbornenes containing either linear or cyclic RGD peptides were synthesized by ring-opening metathesis polymerization (ROMP) using the well-defined ruthenium initiator [(H2IMes)(pyr)2(Cl)2RuCHPh]. The random copolymerization of three separate norbornene monomers allowed for the incorporation of water-soluble polyethylene glycol (PEG) moieties, RGD cell recognition motifs, and primary amines for postpolymerization cross-linking. Following polymer synthesis, thin-film hydrogels were formed by cross-linking with bis(sulfosuccinimidyl) suberate (BS3), and the ability of these materials to support human umbilical vein endothelial cell (HUVEC) adhesion and spreading was evaluated and quantified. When compared to control polymers containing either no peptide or a scrambled RDG peptide, polymers with linear or cyclic RGD at varying concentrations displayed excellent cell adhesive properties in both serum-supplemented and serum-free media. Polymers with cyclic RGD side chains maintained cell adhesion and exhibited comparable integrin binding at a 100-fold lower concentration than those carrying linear RGD peptides. The precise control of monomer incorporation enabled by ROMP allows for quantification of the impact of RGD structure and concentration on cell adhesion and spreading. The results presented here will serve to guide future efforts for the design of RGD functionalized materials with applications in surgery, tissue engineering, and regenerative medicine.

บรรณานุกรม :
Patel, Paresma R. , Kiser, Rosemary Conrad. , Lu, Ying Y. , Fong, Eileen. , Ho, Wilson C. , Tirrell, David A. , Grubbs, Robert H. . (2555). Synthesis and cell adhesive properties of linear and cyclic RGD functionalized polynorbornene thin films..
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Patel, Paresma R. , Kiser, Rosemary Conrad. , Lu, Ying Y. , Fong, Eileen. , Ho, Wilson C. , Tirrell, David A. , Grubbs, Robert H. . 2555. "Synthesis and cell adhesive properties of linear and cyclic RGD functionalized polynorbornene thin films.".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Patel, Paresma R. , Kiser, Rosemary Conrad. , Lu, Ying Y. , Fong, Eileen. , Ho, Wilson C. , Tirrell, David A. , Grubbs, Robert H. . "Synthesis and cell adhesive properties of linear and cyclic RGD functionalized polynorbornene thin films.."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Patel, Paresma R. , Kiser, Rosemary Conrad. , Lu, Ying Y. , Fong, Eileen. , Ho, Wilson C. , Tirrell, David A. , Grubbs, Robert H. . Synthesis and cell adhesive properties of linear and cyclic RGD functionalized polynorbornene thin films.. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.