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Isolating a trimer intermediate in the self-assembly of E2 protein cage.

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Isolating a trimer intermediate in the self-assembly of E2 protein cage.
นักวิจัย : Peng, Tao. , Lee, Hwankyu. , Lim, Sierin.
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Peng, T., Lee, H.,& Lim, S. (2012). Isolating a Trimer Intermediate in the Self-Assembly of E2 Protein Cage. Biomacromolecules, 13(3), 699-705. , http://hdl.handle.net/10220/12755 , http://dx.doi.org/10.1021/bm201587q
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Biomacromolecules
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Understanding the self-assembly mechanism of caged proteins provides clues to develop their potential applications in nanotechnology, such as a nanoscale drug delivery system. The E2 protein from Bacillus stearothermophilus, with a virus-like caged structure, has drawn much attention for its potential application as a nanocapsule. To investigate its self-assembly process from subunits to a spherical protein cage, we truncate the C-terminus of the E2 subunit. The redesigned protein subunit shows dynamic transition between monomer and trimer, but not the integrate 60-mer. The results indicate the role of the trimer as the intermediate and building block during the self-assembly of the E2 protein cage. In combination with the molecular dynamics simulations results, we conclude that the C-terminus modulates the self-assembly of the E2 protein cage from trimer to 60-mer. This investigation elucidates the role of the intersubunit interactions in engineering other functionalities in other caged structure proteins.

บรรณานุกรม :
Peng, Tao. , Lee, Hwankyu. , Lim, Sierin. . (2555). Isolating a trimer intermediate in the self-assembly of E2 protein cage..
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Peng, Tao. , Lee, Hwankyu. , Lim, Sierin. . 2555. "Isolating a trimer intermediate in the self-assembly of E2 protein cage.".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Peng, Tao. , Lee, Hwankyu. , Lim, Sierin. . "Isolating a trimer intermediate in the self-assembly of E2 protein cage.."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Peng, Tao. , Lee, Hwankyu. , Lim, Sierin. . Isolating a trimer intermediate in the self-assembly of E2 protein cage.. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.