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Biophysical properties and supramolecular structure of self-assembled liposome/ε-peptide/DNA nanoparticles : correlation with gene delivery

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Biophysical properties and supramolecular structure of self-assembled liposome/ε-peptide/DNA nanoparticles : correlation with gene delivery
นักวิจัย : Yan, Jiang , Korolev, Nikolay , Eom, Khee Dong , Tam, James P. , Nordenskiöld, Lars
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2554
อ้างอิง : Yan, J., Korolev, N., Eom, K. D., Tam, J. P.,& Nordenskiöld, L. (2012). Biophysical Properties and Supramolecular Structure of Self-Assembled Liposome/ε-Peptide/DNA Nanoparticles: Correlation with Gene Delivery. Biomacromolecules, 13(1), 124-131. , http://hdl.handle.net/10220/12751 , http://dx.doi.org/10.1021/bm201359r
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Biomacromolecules
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Using solid-phase synthesis, lysine can be oligomerized by a reaction of the peptide carboxylate with the ε-amino group to produce nontoxic, biodegradable cationic peptides, ε-oligo(l-lysines). Here α-substituted derivatives of such ε-oligo(l-lysines) containing arginine and histidine in the side chain were tested as vectors for in vitro gene delivery. Combination of ε-oligolysines with the cationic lipid DOTAP and plasmid DNA resulted in transfection efficiency exceeding that of DOTAP alone, without significant increase in cytotoxicity. Synchrotron small-angle X-ray scattering studies revealed self-assembly of the DOTAP, ε-oligolysines, and DNA to ordered lamellar complexes. High transfection efficiency of the nanoparticles correlates with increase in zeta potential above +20 mV and requires particle size to be below 500 nm. The synergistic effect of branched ε-oligolysines and DOTAP in gene delivery can be explained by the increase in surface charge and by the supramolecular structure of the DOTAP/ε-oligolysine/DNA nanoparticles.

บรรณานุกรม :
Yan, Jiang , Korolev, Nikolay , Eom, Khee Dong , Tam, James P. , Nordenskiöld, Lars . (2554). Biophysical properties and supramolecular structure of self-assembled liposome/ε-peptide/DNA nanoparticles : correlation with gene delivery.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Yan, Jiang , Korolev, Nikolay , Eom, Khee Dong , Tam, James P. , Nordenskiöld, Lars . 2554. "Biophysical properties and supramolecular structure of self-assembled liposome/ε-peptide/DNA nanoparticles : correlation with gene delivery".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Yan, Jiang , Korolev, Nikolay , Eom, Khee Dong , Tam, James P. , Nordenskiöld, Lars . "Biophysical properties and supramolecular structure of self-assembled liposome/ε-peptide/DNA nanoparticles : correlation with gene delivery."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2554. Print.
Yan, Jiang , Korolev, Nikolay , Eom, Khee Dong , Tam, James P. , Nordenskiöld, Lars . Biophysical properties and supramolecular structure of self-assembled liposome/ε-peptide/DNA nanoparticles : correlation with gene delivery. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2554.