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Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus
นักวิจัย : Cheng, Ming Soon , Ho, Jia Shin , Tan, Cheong Huat , Wong, Jeslyn Pei Sze , Ng, Lee Ching , Toh, Chee-Seng
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Cheng, M. S., Ho, J. S., Tan, C. H., Wong, J. P. S., Ng, L. C., & Toh, C.-S. (2012). Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus. Analytica Chimica Acta, 725, 74-80. , 0003-2670 , http://hdl.handle.net/10220/12430 , http://dx.doi.org/10.1016/j.aca.2012.03.017
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Analytica chimica acta
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

A sensitive membrane-based electrochemical nanobiosensor is developed for the detection of dengue type 2 virus (DENV-2) using nanoporous alumina-modified platinum electrode. Its sensing mechanism relies on the monitoring of electrode's Faradaic current response toward redox probe, ferrocenemethanol, which is sensitive toward the formation of immune complexes within the alumina nanochannels. Anti-DENV-2 monoclonal antibody (clone 3H5, isotype IgG) is used as the biorecognition element in this work. The stepwise additions of antibody, bovine serum albumin (BSA) and DENV-2 are characterized by differential pulse voltammetry (DPV). A low detection limit of 1 pfu mL−1 with linear range from 1 to 103 pfu mL−1 (R2 = 0.98) can be achieved by the nanobiosensor. The nanobiosensor is selective toward DENV-2 with insignificant cross reaction with non-specific viruses, Chikungunya virus, West Nile virus and dengue type 3 virus (DENV-3). Relative standard deviation (RSD) for triplicate analysis of 5.9% indicates an acceptable level of reproducibility. The first direct quantitation of DENV-2 concentration in whole mosquito vector is demonstrated using this electrochemical nanobiosensor.

บรรณานุกรม :
Cheng, Ming Soon , Ho, Jia Shin , Tan, Cheong Huat , Wong, Jeslyn Pei Sze , Ng, Lee Ching , Toh, Chee-Seng . (2555). Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Cheng, Ming Soon , Ho, Jia Shin , Tan, Cheong Huat , Wong, Jeslyn Pei Sze , Ng, Lee Ching , Toh, Chee-Seng . 2555. "Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Cheng, Ming Soon , Ho, Jia Shin , Tan, Cheong Huat , Wong, Jeslyn Pei Sze , Ng, Lee Ching , Toh, Chee-Seng . "Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Cheng, Ming Soon , Ho, Jia Shin , Tan, Cheong Huat , Wong, Jeslyn Pei Sze , Ng, Lee Ching , Toh, Chee-Seng . Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.