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Characterization of extracellular lignocellulolytic enzymes of Coniochaeta sp. during corn stover bioconversion

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Characterization of extracellular lignocellulolytic enzymes of Coniochaeta sp. during corn stover bioconversion
นักวิจัย : Ravindran, Anita , Adav, Sunil S. , Sze, Siu Kwan
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Ravindran, A., Adav, S. S., & Sze, S. K. (2012). Characterization of extracellular lignocellulolytic enzymes of Coniochaeta sp. during corn stover bioconversion. Process Biochemistry, 47(12), 2440-2448. , 1359-5113 , http://hdl.handle.net/10220/12318 , http://dx.doi.org/10.1016/j.procbio.2012.10.003
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Process biochemistry
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Biorefinery of renewable lignocellulosic biomass to biochemical and biofuel is a promising technology to mitigate global warming and fuel shortage but hydrolysis of recalcitrant lignocellulose to its constitutive components is the bottleneck of the process. This work isolated and characterized a new lignocellulose degrading filamentous fungus from decomposing wood in mangrove area. The strain was identified as Coniochaeta sp. according to ITS rRNA sequences and its phylogenic analysis. The extracellular lignocellulolytic enzymes of this fungal strain, when grown on corn stover, were profiled by LC–MS/MS and exponentially modified protein abundance index (emPAI) based label-free quantitative proteomics approach. We identified 107 potential lignocellulolytic enzymes and their functional classification revealed unique extracellular enzyme system constituting multienzyme complexes of cellulases (29%), hemicellulases (17%), glycoside hydrolases (10%), proteases and peptidases (24%), lignin degrading enzymes (7%) and hypothetical proteins (13%). The growth behavior, biochemical assay and LC–MS/MS analysis of secretome by isolated fungal strain revealed its lignocellulose degradation potential when cultivated with corn stover as a major carbon source.

บรรณานุกรม :
Ravindran, Anita , Adav, Sunil S. , Sze, Siu Kwan . (2555). Characterization of extracellular lignocellulolytic enzymes of Coniochaeta sp. during corn stover bioconversion.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ravindran, Anita , Adav, Sunil S. , Sze, Siu Kwan . 2555. "Characterization of extracellular lignocellulolytic enzymes of Coniochaeta sp. during corn stover bioconversion".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ravindran, Anita , Adav, Sunil S. , Sze, Siu Kwan . "Characterization of extracellular lignocellulolytic enzymes of Coniochaeta sp. during corn stover bioconversion."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Ravindran, Anita , Adav, Sunil S. , Sze, Siu Kwan . Characterization of extracellular lignocellulolytic enzymes of Coniochaeta sp. during corn stover bioconversion. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.