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Direct laser machining-induced topographic pattern promotes up-regulation of myogenic markers in human mesenchymal stem cells

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Direct laser machining-induced topographic pattern promotes up-regulation of myogenic markers in human mesenchymal stem cells
นักวิจัย : Li, Huaqiong , Wen, Feng , Wong, Yee Shan , Boey, Freddy Yin Chiang , Venkatraman, Subbu S. , Leong, David Tai Wei , Ng, Kee Woei , Ng, Gary Ka Lai , Tan, Lay Poh
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2554
อ้างอิง : Li, H., Wen, F., Wong, Y. S., Boey, F. Y. C., Subbu, V. S., Leong, D. T. W., et al. (2012). Direct laser machining-induced topographic pattern promotes up-regulation of myogenic markers in human mesenchymal stem cells. Acta Biomaterialia, 8(2), 531-539. , 1742-7061 , http://hdl.handle.net/10220/12351 , http://dx.doi.org/10.1016/j.actbio.2011.09.029
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Acta biomaterialia
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The engineering of tissue is preferably done with stem cells, which can be differentiated into the tissue of interest using biochemical or physical cues. While much effort has been focused on using biological factors to regulate stem cell differentiation, recently interest in the contribution of physical factors has increased. In this work, three-dimensional (3-D) microchannels with topographic micropatterns were fabricated by femtosecond laser machining on a biodegradable polymer (poly(l-lactide-co-ε-caprolactone)) substrate. Two substrates with narrow and wide channels respectively were created. Human mesenchymal stem cells (hMSCs) were cultured on the scaffolds for cell proliferation and cellular organization. Gene expression and the immunostaining of myogenic and neurogenic markers were studied. Both scaffolds improved the cell alignment along the channels as compared to the control group. Microfilaments within hMSCs were more significantly aligned and elongated on the narrower microchannels. The gene expression study revealed significant up-regulation of several hallmark markers associated with myogenesis for hMSCs cultured on the scaffold with narrow microchannels, while osteogenic and neurogenic markers were down-regulated or remained similar to the control at day 14. Immunostaining of myogen- and neurogen-specific differentiation markers were used to further confirm the specific differentiation towards a myogenic lineage. This study demonstrates that femtosecond laser machining is a versatile tool for generating controllable 3-D microchannels with topographic features that can be used to induce specific myogenic differentiation of hMSCs in vitro, even in the absence of biological factors.

บรรณานุกรม :
Li, Huaqiong , Wen, Feng , Wong, Yee Shan , Boey, Freddy Yin Chiang , Venkatraman, Subbu S. , Leong, David Tai Wei , Ng, Kee Woei , Ng, Gary Ka Lai , Tan, Lay Poh . (2554). Direct laser machining-induced topographic pattern promotes up-regulation of myogenic markers in human mesenchymal stem cells.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Li, Huaqiong , Wen, Feng , Wong, Yee Shan , Boey, Freddy Yin Chiang , Venkatraman, Subbu S. , Leong, David Tai Wei , Ng, Kee Woei , Ng, Gary Ka Lai , Tan, Lay Poh . 2554. "Direct laser machining-induced topographic pattern promotes up-regulation of myogenic markers in human mesenchymal stem cells".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Li, Huaqiong , Wen, Feng , Wong, Yee Shan , Boey, Freddy Yin Chiang , Venkatraman, Subbu S. , Leong, David Tai Wei , Ng, Kee Woei , Ng, Gary Ka Lai , Tan, Lay Poh . "Direct laser machining-induced topographic pattern promotes up-regulation of myogenic markers in human mesenchymal stem cells."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2554. Print.
Li, Huaqiong , Wen, Feng , Wong, Yee Shan , Boey, Freddy Yin Chiang , Venkatraman, Subbu S. , Leong, David Tai Wei , Ng, Kee Woei , Ng, Gary Ka Lai , Tan, Lay Poh . Direct laser machining-induced topographic pattern promotes up-regulation of myogenic markers in human mesenchymal stem cells. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2554.