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Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells
นักวิจัย : Wang, Victor Bochuan , Chua, Song-Lin , Cao, Bin , Seviour, Thomas , Nesatyy, Victor J. , Marsili, Enrico , Kjelleberg, Staffan , Givskov, Michael , Tolker-Nielsen, Tim , Song, Hao , Loo, Say Chye Joachim , Yang, Liang
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : Wang, V. B., Chua, S.-L., Cao, B., Seviour, T., Nesatyy, V. J., Marsili, E., et al. (2013). Engineering PQS Biosynthesis Pathway for Enhancement of Bioelectricity Production in Pseudomonas aeruginosa Microbial Fuel Cells. PLoS ONE, 8(5), e63129. , 1932-6203 , http://hdl.handle.net/10220/11919 , http://dx.doi.org/10.1371/journal.pone.0063129
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : PLoS ONE
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The biosynthesis of the redox shuttle, phenazines, in Pseudomonas aeruginosa, an ubiquitous microorganism in wastewater microflora, is regulated by the 2-heptyl-3,4-dihydroxyquinoline (PQS) quorum-sensing system. However, PQS inhibits anaerobic growth of P. aeruginosa. We constructed a P. aeruginosa strain that produces higher concentrations of phenazines under anaerobic conditions by over-expressing the PqsE effector in a PQS negative ΔpqsC mutant. The engineered strain exhibited an improved electrical performance in microbial fuel cells (MFCs) and potentiostat-controlled electrochemical cells with an approximate five-fold increase of maximum current density relative to the parent strain. Electrochemical analysis showed that the current increase correlates with an over-synthesis of phenazines. These results therefore demonstrate that targeting microbial cell-to-cell communication by genetic engineering is a suitable technique to improve power output of bioelectrochemical systems.

บรรณานุกรม :
Wang, Victor Bochuan , Chua, Song-Lin , Cao, Bin , Seviour, Thomas , Nesatyy, Victor J. , Marsili, Enrico , Kjelleberg, Staffan , Givskov, Michael , Tolker-Nielsen, Tim , Song, Hao , Loo, Say Chye Joachim , Yang, Liang . (2556). Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Wang, Victor Bochuan , Chua, Song-Lin , Cao, Bin , Seviour, Thomas , Nesatyy, Victor J. , Marsili, Enrico , Kjelleberg, Staffan , Givskov, Michael , Tolker-Nielsen, Tim , Song, Hao , Loo, Say Chye Joachim , Yang, Liang . 2556. "Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Wang, Victor Bochuan , Chua, Song-Lin , Cao, Bin , Seviour, Thomas , Nesatyy, Victor J. , Marsili, Enrico , Kjelleberg, Staffan , Givskov, Michael , Tolker-Nielsen, Tim , Song, Hao , Loo, Say Chye Joachim , Yang, Liang . "Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2556. Print.
Wang, Victor Bochuan , Chua, Song-Lin , Cao, Bin , Seviour, Thomas , Nesatyy, Victor J. , Marsili, Enrico , Kjelleberg, Staffan , Givskov, Michael , Tolker-Nielsen, Tim , Song, Hao , Loo, Say Chye Joachim , Yang, Liang . Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in pseudomonas aeruginosa microbial fuel cells. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2556.