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Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics
นักวิจัย : Shi, Haibin , Kwok, Ryan T. K. , Liu, Jianzhao , Xing, Bengang , Tang, Ben Zhong , Liu, Bin
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Shi, H., Kwok, R. T. K., Liu, J., Xing, B., Tang, B. Z.,& Liu, B. (2012). Real-Time Monitoring of Cell Apoptosis and Drug Screening Using Fluorescent Light-Up Probe with Aggregation-Induced Emission Characteristics. Journal of the American Chemical Society, 134(43), 17972-17981. , http://hdl.handle.net/10220/11235 , http://dx.doi.org/10.1021/ja3064588
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of the American chemical society
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Real-time monitoring of cell apoptosis could provide valuable insights into early detection of therapy efficiency and evaluation of disease progression. In this work, we designed and synthesized a new live-cell-permeable, fluorescent light-up probe for real-time cell apoptosis imaging. The probe is comprised of a hydrophilic caspase-specific Asp-Glu-Val-Asp (DEVD) peptide and a hydrophobic tetraphenylethene (TPE) unit, a typical fluorogen with aggregation-induced emission characteristics. In aqueous solution, the probe is almost nonfluorescent but displays significant fluorescence enhancement in response to caspase-3/-7, which are activated in the apoptotic process and able to cleave the DEVD moieties. This fluorescence “turn-on” response is ascribed to aggregation of cleaved hydrophobic TPE residues, which restricts the intramolecular rotations of TPE phenyl rings and populates the radiative decay channels. The light-up nature of the probe allows real-time monitoring of caspase-3/-7 activities both in solutions and in living cells with a high signal-to-noise ratio. The probe provides a new opportunity to screen enzyme inhibitors and evaluate the apoptosis-associated drug efficacy.

บรรณานุกรม :
Shi, Haibin , Kwok, Ryan T. K. , Liu, Jianzhao , Xing, Bengang , Tang, Ben Zhong , Liu, Bin . (2555). Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Shi, Haibin , Kwok, Ryan T. K. , Liu, Jianzhao , Xing, Bengang , Tang, Ben Zhong , Liu, Bin . 2555. "Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Shi, Haibin , Kwok, Ryan T. K. , Liu, Jianzhao , Xing, Bengang , Tang, Ben Zhong , Liu, Bin . "Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Shi, Haibin , Kwok, Ryan T. K. , Liu, Jianzhao , Xing, Bengang , Tang, Ben Zhong , Liu, Bin . Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.