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Electrochemically amplified molecular beacon biosensor for ultrasensitive DNA sequence-specific detection of Legionella sp

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Electrochemically amplified molecular beacon biosensor for ultrasensitive DNA sequence-specific detection of Legionella sp
นักวิจัย : Rai, Varun , Nyine, Yin Thu , Hapuarachchi, Hapuarachchige C. , Yap, Hooi Ming , Ng, Lee Ching , Toh, Chee-Seng
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2554
อ้างอิง : Rai, V., Nyine, Y. T., Hapuarachchi, H. C., Yap, H. M., Ng, L. C., Toh, C.-S. (2011). Electrochemically amplified molecular beacon biosensor for ultrasensitive DNA sequence-specific detection of Legionella sp. Biosensors and Bioelectronics, 32(1), 133-140. , http://hdl.handle.net/10220/11077 , http://dx.doi.org/10.1016/j.bios.2011.11.046
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Biosensors and bioelectronics
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

An electrochemically amplified molecular beacon (EAMB) biosensor is constructed using thiolated hairpin DNA-ferrocene probes on gold electrode. The switching from “on” to “off” states of individual probes in the presence of complementary DNA target influences the electrode potential, besides the current, owing to changes in surface density of the electroactive hairpin DNA-ferrocene probes. The EAMB biosensor demonstrates linear range over 8 orders of magnitude with ultrasensitive detection limit of 2.3 × 10−14 M for the quantification of a 21-mer DNA sequence. Its applicability is tested against PCR amplicons derived from genomic DNA of live Legionella pneumophila. Excellent specificity down to one and three nucleotides mismatches in another strain of L. pneumophila and a different bacterium species, respectively, is demonstrated.

บรรณานุกรม :
Rai, Varun , Nyine, Yin Thu , Hapuarachchi, Hapuarachchige C. , Yap, Hooi Ming , Ng, Lee Ching , Toh, Chee-Seng . (2554). Electrochemically amplified molecular beacon biosensor for ultrasensitive DNA sequence-specific detection of Legionella sp.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Rai, Varun , Nyine, Yin Thu , Hapuarachchi, Hapuarachchige C. , Yap, Hooi Ming , Ng, Lee Ching , Toh, Chee-Seng . 2554. "Electrochemically amplified molecular beacon biosensor for ultrasensitive DNA sequence-specific detection of Legionella sp".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Rai, Varun , Nyine, Yin Thu , Hapuarachchi, Hapuarachchige C. , Yap, Hooi Ming , Ng, Lee Ching , Toh, Chee-Seng . "Electrochemically amplified molecular beacon biosensor for ultrasensitive DNA sequence-specific detection of Legionella sp."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2554. Print.
Rai, Varun , Nyine, Yin Thu , Hapuarachchi, Hapuarachchige C. , Yap, Hooi Ming , Ng, Lee Ching , Toh, Chee-Seng . Electrochemically amplified molecular beacon biosensor for ultrasensitive DNA sequence-specific detection of Legionella sp. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2554.