ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Fluorescence-based reporter for gauging cyclic Di-GMP levels in Pseudomonas aeruginosa

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Fluorescence-based reporter for gauging cyclic Di-GMP levels in Pseudomonas aeruginosa
นักวิจัย : Rybtke, Morten Theil , Borlee, Bradley R. , Murakami, Keiji , Irie, Yasuhiko , Hentzer, Morten , Nielsen, Thomas E. , Givskov, Michael , Parsek, Matthew R. , Tolker-Nielsen, Tim
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Rybtke, M. T., Borlee, B. R., Murakami, K., Irie, Y., Hentzer, M., Nielsen, T. E., et al. (2012). Fluorescence-based reporter for gauging cyclic Di-GMP levels in Pseudomonas aeruginosa. Applied and Environmental Microbiology, 78(15), 5060-5069. , 0099-2240 , http://hdl.handle.net/10220/10971 , http:dx.doi.org/10.1128/AEM.00414-12
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Applied and environmental microbiology
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The increased tolerance toward the host immune system and antibiotics displayed by biofilm-forming Pseudomonas aeruginosa and other bacteria in chronic infections such as cystic fibrosis bronchopneumonia is of major concern. Targeting of biofilm formation is believed to be a key aspect in the development of novel antipathogenic drugs that can augment the effect of classic antibiotics by decreasing antimicrobial tolerance. The second messenger cyclic di-GMP is a positive regulator of biofilm formation, and cyclic di-GMP signaling is now regarded as a potential target for the development of antipathogenic compounds. Here we describe the development of fluorescent monitors that can gauge the cellular level of cyclic di-GMP in P. aeruginosa. We have created cyclic di-GMP level reporters by transcriptionally fusing the cyclic di-GMP-responsive cdrA promoter to genes encoding green fluorescent protein. We show that the reporter constructs give a fluorescent readout of the intracellular level of cyclic di-GMP in P. aeruginosa strains with different levels of cyclic di-GMP. Furthermore, we show that the reporters are able to detect increased turnover of cyclic di-GMP mediated by treatment of P. aeruginosa with the phosphodiesterase inducer nitric oxide. Considering that biofilm formation is a necessity for the subsequent development of a chronic infection and therefore a pathogenicity trait, the reporters display a significant potential for use in the identification of novel antipathogenic compounds targeting cyclic di-GMP signaling, as well as for use in research aiming at understanding the biofilm biology of P. aeruginosa.

บรรณานุกรม :
Rybtke, Morten Theil , Borlee, Bradley R. , Murakami, Keiji , Irie, Yasuhiko , Hentzer, Morten , Nielsen, Thomas E. , Givskov, Michael , Parsek, Matthew R. , Tolker-Nielsen, Tim . (2555). Fluorescence-based reporter for gauging cyclic Di-GMP levels in Pseudomonas aeruginosa.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Rybtke, Morten Theil , Borlee, Bradley R. , Murakami, Keiji , Irie, Yasuhiko , Hentzer, Morten , Nielsen, Thomas E. , Givskov, Michael , Parsek, Matthew R. , Tolker-Nielsen, Tim . 2555. "Fluorescence-based reporter for gauging cyclic Di-GMP levels in Pseudomonas aeruginosa".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Rybtke, Morten Theil , Borlee, Bradley R. , Murakami, Keiji , Irie, Yasuhiko , Hentzer, Morten , Nielsen, Thomas E. , Givskov, Michael , Parsek, Matthew R. , Tolker-Nielsen, Tim . "Fluorescence-based reporter for gauging cyclic Di-GMP levels in Pseudomonas aeruginosa."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Rybtke, Morten Theil , Borlee, Bradley R. , Murakami, Keiji , Irie, Yasuhiko , Hentzer, Morten , Nielsen, Thomas E. , Givskov, Michael , Parsek, Matthew R. , Tolker-Nielsen, Tim . Fluorescence-based reporter for gauging cyclic Di-GMP levels in Pseudomonas aeruginosa. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.