ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Expression and purification of coronavirus envelope proteins using a modified β-barrel construct

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Expression and purification of coronavirus envelope proteins using a modified β-barrel construct
นักวิจัย : Parthasarathy, Krupakar , Lu, Huang , Surya, Wahyu , Vararattanavech, Ardcharaporn , Pervushin, Konstantin , Torres, Jaume
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Parthasarathy, K., Lu, H., Surya, W., Vararattanavech, A., Pervushin, K., & Torres, J. (2012). Expression and purification of coronavirus envelope proteins using a modified β-barrel construct. Protein Expression and Purification, 85(1), 133–141. , 1046-5928 , http://hdl.handle.net/10220/10981
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Protein expression and purification
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Coronavirus envelope (E) proteins are short (∼100 residues) polypeptides that contain at least one transmembrane (TM) domain and a cluster of 2–3 juxtamembrane cysteines. These proteins are involved in viral morphogenesis and tropism, and their absence leads in some cases to aberrant virions, or to viral attenuation. In common to other viroporins, coronavirus envelope proteins increase membrane permeability to ions. Although an NMR-based model for the TM domain of the E protein in the severe acute respiratory syndrome virus (SARS-CoV E) has been reported, structural data and biophysical studies of full length E proteins are not available because efficient expression and purification methods for these proteins are lacking. Herein we have used a novel fusion protein consisting of a modified β-barrel to purify both wild type and cysteine-less mutants of two representatives of coronavirus E proteins: the shortest (76 residues), from SARS-CoV E, and one of the longest (109 residues), from the infectious bronchitis virus (IBV E). The fusion construct was subsequently cleaved with cyanogen bromide and all polypeptides were obtained with high purity. This is an approach that can be used in other difficult hydrophobic peptides.

บรรณานุกรม :
Parthasarathy, Krupakar , Lu, Huang , Surya, Wahyu , Vararattanavech, Ardcharaporn , Pervushin, Konstantin , Torres, Jaume . (2555). Expression and purification of coronavirus envelope proteins using a modified β-barrel construct.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Parthasarathy, Krupakar , Lu, Huang , Surya, Wahyu , Vararattanavech, Ardcharaporn , Pervushin, Konstantin , Torres, Jaume . 2555. "Expression and purification of coronavirus envelope proteins using a modified β-barrel construct".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Parthasarathy, Krupakar , Lu, Huang , Surya, Wahyu , Vararattanavech, Ardcharaporn , Pervushin, Konstantin , Torres, Jaume . "Expression and purification of coronavirus envelope proteins using a modified β-barrel construct."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Parthasarathy, Krupakar , Lu, Huang , Surya, Wahyu , Vararattanavech, Ardcharaporn , Pervushin, Konstantin , Torres, Jaume . Expression and purification of coronavirus envelope proteins using a modified β-barrel construct. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.