ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

A universal description for the experimental behavior of salt-(in)dependent oligocation-induced DNA condensation

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : A universal description for the experimental behavior of salt-(in)dependent oligocation-induced DNA condensation
นักวิจัย : Korolev, Nikolay , Berezhnoy, Nikolay V. , Eom, Khee Dong , Tam, James P. , Nordenskiöld, Lars
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2552
อ้างอิง : Korolev, N., Berezhnoy, N. V., Eom, K. D., Tam, J. P., & Nordenskiöld, L. (2012). A universal description for the experimental behavior of salt-(in)dependent oligocation-induced DNA condensation. Nucleic Acids Research, 37(21), 7137-7150. , 0305-1048 , http://hdl.handle.net/10220/10918 , http://dx.doi.org/10.1093/nar/gkp683
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Nucleic acids research
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

We report a systematic study of the condensation of plasmid DNA by oligocations with variation of the charge, Z, from +3 to +31. The oligocations include a series of synthetic linear ε-oligo(l-lysines), (denoted εKn, n = 3–10, 31; n is the number of lysines equal to the ligand charge) and branched α-substituted homologues of εK10: εYK10, εLK10 (Z = +10); εRK10, εYRK10 and εLYRK10 (Z = +20). Data were obtained by light scattering, UV absorption monitored precipitation assay and isothermal titration calorimetry in a wide range concentrations of DNA and monovalent salt (KCl, CKCl). The dependence of EC50 (ligand concentration at the midpoint of DNA condensation) on CKCl shows the existence of a salt-independent regime at low CKCl and a salt-dependent regime with a steep rise of EC50 with increase of CKCl. Increase of the ligand charge shifts the transition from the salt-independent to salt-dependent regime to higher CKCl. A novel and simple relationship describing the EC50 dependence on DNA concentration, charge of the ligand and the salt-dependent dissociation constant of the ligand–DNA complex is derived. For the ε-oligolysines εK3–εK10, the experimental dependencies of EC50 on CKCl and Z are well-described by an equation with a common set of parameters. Implications from our findings for understanding DNA condensation in chromatin are discussed.

บรรณานุกรม :
Korolev, Nikolay , Berezhnoy, Nikolay V. , Eom, Khee Dong , Tam, James P. , Nordenskiöld, Lars . (2552). A universal description for the experimental behavior of salt-(in)dependent oligocation-induced DNA condensation.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Korolev, Nikolay , Berezhnoy, Nikolay V. , Eom, Khee Dong , Tam, James P. , Nordenskiöld, Lars . 2552. "A universal description for the experimental behavior of salt-(in)dependent oligocation-induced DNA condensation".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Korolev, Nikolay , Berezhnoy, Nikolay V. , Eom, Khee Dong , Tam, James P. , Nordenskiöld, Lars . "A universal description for the experimental behavior of salt-(in)dependent oligocation-induced DNA condensation."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2552. Print.
Korolev, Nikolay , Berezhnoy, Nikolay V. , Eom, Khee Dong , Tam, James P. , Nordenskiöld, Lars . A universal description for the experimental behavior of salt-(in)dependent oligocation-induced DNA condensation. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2552.