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An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE
นักวิจัย : Yu, Yong , Qin, Anjun , Feng, Chao , Lu, Ping , Ng, Ka Ming , Luo, Kathy Qian , Tang, Ben Zhong
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Yu, Y., Qin, A., Feng, C., Lu, P., Ng, K. M., Luo, K. Q., et al. (2012). An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE. Analyst, 137(23), 5592-5596. , 0003-2654 , http://hdl.handle.net/10220/10751 , http://dx.doi.org/10.1039/c2an36002k
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Analyst
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

A new aggregation-induced emission (AIE) compound 1,2-bis[4-(isothiocyanatomethyl)phenyl]-1,2-diphenylethene (2) was synthesized for use in SDS-PAGE. The molecule is practically nonemissive in solution but becomes highly emissive after reacting with the amine groups of the proteins by either the prestaining or poststaining method. The sensitivity of 2 achieved in the prestaining method is the same as that of Coomassie brilliant blue (CBB), while that observed in the poststaining method is higher than that of CBB. Excellent linear responses with the amount of protein were obtained in both cases. The detection of a mixture of proteins with different molecular weights was successfully achieved.

บรรณานุกรม :
Yu, Yong , Qin, Anjun , Feng, Chao , Lu, Ping , Ng, Ka Ming , Luo, Kathy Qian , Tang, Ben Zhong . (2555). An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Yu, Yong , Qin, Anjun , Feng, Chao , Lu, Ping , Ng, Ka Ming , Luo, Kathy Qian , Tang, Ben Zhong . 2555. "An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Yu, Yong , Qin, Anjun , Feng, Chao , Lu, Ping , Ng, Ka Ming , Luo, Kathy Qian , Tang, Ben Zhong . "An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Yu, Yong , Qin, Anjun , Feng, Chao , Lu, Ping , Ng, Ka Ming , Luo, Kathy Qian , Tang, Ben Zhong . An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.