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Structural basis of RNA binding by leucine zipper GCN4

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Structural basis of RNA binding by leucine zipper GCN4
นักวิจัย : Nikolaev, Yaroslav , Pervushin, Konstantin
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Nikolaev, Y., & Pervushin, K. (2012). Structural basis of RNA binding by leucine zipper GCN4. Protein Science, 21(5), 667-676. , 0961-8368 , http://hdl.handle.net/10220/10556 , http://dx.doi.org/10.1002/pro.2051
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Protein science
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Recently, we showed that leucine zipper (LZ) motifs of basic leucine zipper (bZIP) transcription factors GCN4 and c-Jun are capable of catalyzing degradation of RNA (Nikolaev et al., PLoS ONE 2010; 5:e10765). This observation is intriguing given the tight regulation of RNA turnover control and the antiquity of bZIP transcription factors. To support further mechanistic studies, herein, we elucidated RNA binding interface of the GCN4 leucine zipper motif from yeast. Solution NMR experiments showed that the LZ-RNA interaction interface is located in the first two heptads of LZ moiety, and that only the dimeric (coiled coil) LZ conformation is capable of binding RNA. Site-directed mutagenesis of the LZ-GCN4 RNA binding interface showed that substrate binding is facilitated by lysine and arginine side chains, and that at least one nucleophilic residue is located in proximity to the RNA phosphate backbone. Further studies in the context of full-length bZIP factors are envisaged to address the biological relevance of LZ RNase activity.

บรรณานุกรม :
Nikolaev, Yaroslav , Pervushin, Konstantin . (2555). Structural basis of RNA binding by leucine zipper GCN4.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Nikolaev, Yaroslav , Pervushin, Konstantin . 2555. "Structural basis of RNA binding by leucine zipper GCN4".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Nikolaev, Yaroslav , Pervushin, Konstantin . "Structural basis of RNA binding by leucine zipper GCN4."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Nikolaev, Yaroslav , Pervushin, Konstantin . Structural basis of RNA binding by leucine zipper GCN4. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.