ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers
นักวิจัย : Bao, Hongqian , Ping, Yuan , Pan, Yongzheng , Li, Lin , Li, Jun , Gan, Leong Huat
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Bao, H., Ping, Y., Pan, Y., Li, L., Li, J., & Gan, L. H. (2012). Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers. Soft Matter, 8(8), 2518-2526. , 1744-683X , http://hdl.handle.net/10220/10348 , http://dx.doi.org/10.1039/c2sm07083a
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Soft matter
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Serial thermo-sensitive CS(-g-PDMAEMA)-g-PNIPAM terpolymers (termed as TCS) were prepared by atom transfer radical polymerization (ATRP) and click reactions, where CS, PDMAEMA, and PNIPAM stand for chitosan, poly((2-dimethylamino)ethyl methacrylate), and poly(N-isopropylacrylamide) respectively. Their lower critical solution temperature (LCST) determined by laser light scattering (LLS) was around 32.5–35.1 °C. The incorporation of CS and PNIPAM considerably decreased the cytotoxicity of PDMAEMA. Gel electrophoresis and TEM results revealed that the association and dissociation of TCS/DNA complexes could be tuned by varying temperature. The transfection level of TCS and controls was evaluated with COS7 and HeLa cells using two different reporter genes, pRL-CMV encoding luciferase and pEGFP-N1 encoding green fluorescence protein (GFP). The transfection efficiency of one specific terpolymer (TCS4) incubated at 37 °C for 22 h, 20 °C for 2 h and 37 °C for 24 h increased 1–4 fold compared to that incubated at 37 °C for 48 h. Encouragingly, at optimum N/P ratios, the transfection efficiency of TCS was comparable or superior to that of “gold-standard” PEI (25 kDa).

บรรณานุกรม :
Bao, Hongqian , Ping, Yuan , Pan, Yongzheng , Li, Lin , Li, Jun , Gan, Leong Huat . (2555). Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Bao, Hongqian , Ping, Yuan , Pan, Yongzheng , Li, Lin , Li, Jun , Gan, Leong Huat . 2555. "Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Bao, Hongqian , Ping, Yuan , Pan, Yongzheng , Li, Lin , Li, Jun , Gan, Leong Huat . "Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Bao, Hongqian , Ping, Yuan , Pan, Yongzheng , Li, Lin , Li, Jun , Gan, Leong Huat . Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.