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Investigating the influence of ionic concentrations and subunit interactions on the self-assembly of E2 protein

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Investigating the influence of ionic concentrations and subunit interactions on the self-assembly of E2 protein
นักวิจัย : Peng, Tao , Tan, Sze Wah , Dharmawan, Ratna Ekawati , Lim, Sierin
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Peng, T., Tan, S. W., Dharmawan, R. E., & Lim, S. (2012). Investigating the Influence of Ionic Concentrations and Subunit Interactions on the Self-Assembly of E2 Protein. International Conference on Nanotechnology - Research and Commercialization 2011, 1502, 34-52. , http://hdl.handle.net/10220/10105 , http://dx.doi.org/10.1063/1.4769132
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Understanding of self-assembly mechanism of viruslike protein cage is important in controlling release of molecular cargo for applications in drug delivery. E2 core protein is composed of 60 subunits which self-assemble into a hollow 25-nm porous protein cage. Due to its virus like dodecahedral structure without infectious capacity, we are interested in its potential application as nanocapsule in drug delivery. In our study, extrinsic and intrinsic factors that influence self-assembly were evaluated. Extrinsic factors, such as salts and denaturants, were introduced into E2 protein solution. The hydrodynamic diameter of the E2 core protein was used to monitor its disassembly or aggregation. We found that the protein size increased proportionally with the salt concentration while the size decreased as the denaturant concentration increased. To assess intrinsic factors that influence E2 self-assembly, we identified some key amino acids at interfaces of subunits and performed site-directed mutagenesis on them. Characterizations of each mutant for size and secondary structure contents were performed. We found that mutations at the inner surface have no apparent effects on both protein sizes and secondary structures. The mutations at intra-trimer interfaces changed the secondary structure contents but the protein sizes remained stable.

บรรณานุกรม :
Peng, Tao , Tan, Sze Wah , Dharmawan, Ratna Ekawati , Lim, Sierin . (2555). Investigating the influence of ionic concentrations and subunit interactions on the self-assembly of E2 protein.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Peng, Tao , Tan, Sze Wah , Dharmawan, Ratna Ekawati , Lim, Sierin . 2555. "Investigating the influence of ionic concentrations and subunit interactions on the self-assembly of E2 protein".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Peng, Tao , Tan, Sze Wah , Dharmawan, Ratna Ekawati , Lim, Sierin . "Investigating the influence of ionic concentrations and subunit interactions on the self-assembly of E2 protein."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Peng, Tao , Tan, Sze Wah , Dharmawan, Ratna Ekawati , Lim, Sierin . Investigating the influence of ionic concentrations and subunit interactions on the self-assembly of E2 protein. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.