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Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications
นักวิจัย : Mishra, Biswajit , Basu, Anindya , Saravanan, Rathi , Xiang, Li , Yang, Lim Kai , Leong, Susanna Su Jan
คำค้น : DRNTU::Science::Biological sciences::Microbiology::Bacteria
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : Mishra, B., Basu, A., Saravanan, R., Xiang, L., Yang, L. K., & Leong, S. S. J. (2013). Lasioglossin-III: antimicrobial characterization and feasibility study for immobilization applications. RSC Advances, 3(24), 9534-9543. , http://hdl.handle.net/10220/10057 , http://dx.doi.org/10.1039/C3RA40887F , 171651
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : RSC advances
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Prevalent bacterial colonization and subsequent biofilm formation in biomedical implants demand for improved antimicrobial properties of these devices. To address this problem, immobilizing antimicrobial peptides (AMPs) on implants is a promising solution because of their biocompatibility and lesser likelihood to incur pathogen resistance. This study presents a systematic approach towards evaluating the feasibility of Lasioglossin-III (Lasio-III) (a new bee venom AMP, found in Lasioglossum laticeps) to be tethered onto biodevices. Antimicrobial characterization of Lasio-III in solution confirms the peptide’s membranolytic mode of action and its salt-resistant, broad antimicrobial spectrum activity and anti-biofilm properties against Gram negative and Gram positive bacteria. Lassio-III was covalently immobilized on silicon surfaces using APTES and PEG spacers of varying lengths. Surface characterization of the AMP-immobilized silicon was done using water contact angle measurements, XPS analysis and ellipsometry. Even at modest surface peptide concentrations of y180 ng cm22 , Lassio-III showed antibacterial activities which were further enhanced with increasing PEG spacer lengths, as determined by live CFU counting and ATP leakage experiments. This proof-of-concept study demonstrates the potential of Lasio-III as an antimicrobial coating candidate.

บรรณานุกรม :
Mishra, Biswajit , Basu, Anindya , Saravanan, Rathi , Xiang, Li , Yang, Lim Kai , Leong, Susanna Su Jan . (2556). Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Mishra, Biswajit , Basu, Anindya , Saravanan, Rathi , Xiang, Li , Yang, Lim Kai , Leong, Susanna Su Jan . 2556. "Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Mishra, Biswajit , Basu, Anindya , Saravanan, Rathi , Xiang, Li , Yang, Lim Kai , Leong, Susanna Su Jan . "Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2556. Print.
Mishra, Biswajit , Basu, Anindya , Saravanan, Rathi , Xiang, Li , Yang, Lim Kai , Leong, Susanna Su Jan . Lasioglossin-III : antimicrobial characterization and feasibility study for immobilization applications. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2556.