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Argonaute identity defines the length of mature mammalian microRNAs

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Argonaute identity defines the length of mature mammalian microRNAs
นักวิจัย : Juvvuna, Prasanna Kumar , Khandelia, Piyush , Lee, Li Ming , Makeyev, Eugene V.
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Juvvuna, P. K., Khandelia, P., Lee, L. M., & Makeyev, E. V. (2012). Argonaute identity defines the length of mature mammalian microRNAs. Nucleic Acids Research, 40(14), 6808-6820. , http://hdl.handle.net/10220/10002 , http://dx.doi.org/10.1093/nar/gks293
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Nucleic acids research
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

MicroRNAs (miRNAs) are 19- to 25-nt-long non-coding RNAs that regulate gene expression by base-pairing with target mRNAs and reducing their stability or translational efficiency. Mammalian miRNAs function in association with four closely related Argonaute proteins, AGO1–4. All four proteins contain the PAZ and the MID domains interacting with the miRNA 30 and 50 termini, respectively, as well as the PIWI domain comprising an mRNA ‘slicing’ activity in the case of AGO2 but not AGO1, AGO3 and AGO4. However, the slicing mode of the miRNA-programmed AGO2 is rarely realized in vivo and the four Argonautes are thought to play largely overlapping roles in the mammalian miRNA pathway. Here, we show that the average length of many miRNAs is diminished during nervous system development as a result of progressive shortening of the miRNA 30 ends. We link this modification with an increase in the fractional abundance of Ago2 in the adult brain and identify a specific structural motif within the PAZ domain that enables efficient trimming of miRNAs associated with this but not the other three Argonautes. Taken together, our data suggest that mammalian Argonautes may define the length and possibly biological activity of mature mammalian miRNAs in a developmentally controlled manner.

บรรณานุกรม :
Juvvuna, Prasanna Kumar , Khandelia, Piyush , Lee, Li Ming , Makeyev, Eugene V. . (2555). Argonaute identity defines the length of mature mammalian microRNAs.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Juvvuna, Prasanna Kumar , Khandelia, Piyush , Lee, Li Ming , Makeyev, Eugene V. . 2555. "Argonaute identity defines the length of mature mammalian microRNAs".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Juvvuna, Prasanna Kumar , Khandelia, Piyush , Lee, Li Ming , Makeyev, Eugene V. . "Argonaute identity defines the length of mature mammalian microRNAs."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Juvvuna, Prasanna Kumar , Khandelia, Piyush , Lee, Li Ming , Makeyev, Eugene V. . Argonaute identity defines the length of mature mammalian microRNAs. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.