ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Imaging beads-retained prey assay for rapid and quantitative protein-protein interaction

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Imaging beads-retained prey assay for rapid and quantitative protein-protein interaction
นักวิจัย : Zhou, Y. , Hong, Wanjin , Lu, Lei
คำค้น : DRNTU::Science::Biological sciences
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : Zhou, Y., Hong, W., & Lu, L. (2013). Imaging Beads-Retained Prey Assay for Rapid and Quantitative Protein-Protein Interaction. PLoS ONE, 8(3). , 1932-6203 , http://hdl.handle.net/10220/9876 , http://dx.doi.org/10.1371/journal.pone.0059727
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : PLoS ONE
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Conventional Western blot based pull-down methods involve lengthy and laborious work and the results are generally not quantitative. Here, we report the imaging beads-retained prey (IBRP) assay that is rapid and quantitative in studying protein-protein interactions. In this assay, the bait is immobilized onto beads and the prey is fused with a fluorescence protein. The assay takes advantage of the fluorescence of prey and directly quantifies the amount of prey binding to the immobilized bait under a microscope. We validated the assay using previously well studied interactions and found that the amount of prey retained on beads could have a relative linear relationship to both the inputs of bait and prey. IBRP assay provides a universal, fast, quantitative and economical method to study protein interactions and it could be developed to a medium- or high-throughput compatible method. With the availability of fluorescence tagged whole genome ORFs in several organisms, we predict IBRP assay should have wide applications.

บรรณานุกรม :
Zhou, Y. , Hong, Wanjin , Lu, Lei . (2556). Imaging beads-retained prey assay for rapid and quantitative protein-protein interaction.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Zhou, Y. , Hong, Wanjin , Lu, Lei . 2556. "Imaging beads-retained prey assay for rapid and quantitative protein-protein interaction".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Zhou, Y. , Hong, Wanjin , Lu, Lei . "Imaging beads-retained prey assay for rapid and quantitative protein-protein interaction."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2556. Print.
Zhou, Y. , Hong, Wanjin , Lu, Lei . Imaging beads-retained prey assay for rapid and quantitative protein-protein interaction. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2556.