ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Silencing of diphthamide synthesis 3 (Dph3) reduces metastasis of murine melanoma

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Silencing of diphthamide synthesis 3 (Dph3) reduces metastasis of murine melanoma
นักวิจัย : Wang, Lei , Shi, Yu , Ju, Peijun , Liu, Rui , Yeo, Siok Ping , Xia, Yinyan , Owlanj, Hamed , Feng, Zhiwei
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Wang, L., Shi, Y., Ju, P., Liu, R., Yeo, S. P., Xia, Y., et al. (2012). Silencing of Diphthamide Synthesis 3 (Dph3) Reduces Metastasis of Murine Melanoma. PLoS ONE, 7(11). , 1932-6203 , http://hdl.handle.net/10220/9347 , http://dx.doi.org/10.1371/journal.pone.0049988
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : PLoS ONE
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Melanoma is the most dangerous skin cancer due to its highly metastatic potential and resistance to chemotherapy. Currently, there is no effective treatment for melanoma once it is progressed to metastatic stage. Therefore, further study to elucidate the molecular mechanism underlying the metastasis of melanoma cells is urgently required for the improvement of melanoma treatment. In the present study, we found that diphthamide synthesis 3 (Dph3) is involved in the metastasis of B16F10 murine melanoma cells by insertional mutagenesis. We demonstrated that Dph3 disruption impairs the migration of B16F10 murine melanoma cells. The requirement of Dph3 in the migration of melanoma cells was further confirmed by gene silencing with siRNA in vitro. In corresponding to this result, overexpression of Dph3 significantly promoted the migratory ability of B16F10 and B16F0 melanoma cells. Moreover, down regulation of Dph3 expression in B16F10 melanoma cells strikingly inhibits their cellular invasion and metastasis in vivo. Finally, we found that Dph3 promotes melanoma migration and invasion through the AKT signaling pathway. To conclude, our findings suggest a novel mechanism underlying the metastasis of melanoma cells which might serve as a new intervention target for the treatment of melanoma.

บรรณานุกรม :
Wang, Lei , Shi, Yu , Ju, Peijun , Liu, Rui , Yeo, Siok Ping , Xia, Yinyan , Owlanj, Hamed , Feng, Zhiwei . (2555). Silencing of diphthamide synthesis 3 (Dph3) reduces metastasis of murine melanoma.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Wang, Lei , Shi, Yu , Ju, Peijun , Liu, Rui , Yeo, Siok Ping , Xia, Yinyan , Owlanj, Hamed , Feng, Zhiwei . 2555. "Silencing of diphthamide synthesis 3 (Dph3) reduces metastasis of murine melanoma".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Wang, Lei , Shi, Yu , Ju, Peijun , Liu, Rui , Yeo, Siok Ping , Xia, Yinyan , Owlanj, Hamed , Feng, Zhiwei . "Silencing of diphthamide synthesis 3 (Dph3) reduces metastasis of murine melanoma."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Wang, Lei , Shi, Yu , Ju, Peijun , Liu, Rui , Yeo, Siok Ping , Xia, Yinyan , Owlanj, Hamed , Feng, Zhiwei . Silencing of diphthamide synthesis 3 (Dph3) reduces metastasis of murine melanoma. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.