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Crystallization and preliminary X-ray diffraction characterization of the XccFimXEAL-c-di-GMP and XccFimXEAL-c-di-GMP-XccPilZ complexes from Xanthomonas campestris

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Crystallization and preliminary X-ray diffraction characterization of the XccFimXEAL-c-di-GMP and XccFimXEAL-c-di-GMP-XccPilZ complexes from Xanthomonas campestris
นักวิจัย : Liao, Yi-Ting , Chin, Ko-Hsin , Kuo, Wei-Ting , Chuah, Mary Lay-Cheng , Liang, Zhao-Xun , Chou, Shan-Ho
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Liao, Y.- T., Chin, K.- H., Kuo, W.- T., Chuah, M. L.- C., Liang, Z.- X., & Chou, S.- H. (2012). Crystallization and preliminary X-ray diffraction characterization of the XccFimXEAL-c-di-GMP and XccFimXEAL-c-di-GMP-XccPilZ complexes from Xanthomonas campestris. Acta Crystallographica Section F Structural Biology and Crystallization Communications, 68(3), 301-305. , 1744-3091 , http://hdl.handle.net/10220/9183 , http://dx.doi.org/10.1107/S1744309112000590
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Acta crystallographica section F Structural Biology and Crystallization Communications
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

c-di-GMP is a major secondary-messenger molecule in regulation of bacterial pathogenesis. Therefore, the c-di-GMP-mediated signal transduction network is of considerable interest. The PilZ domain was the first c-di-GMP receptor to be predicted and identified. However, every PilZ domain binds c-di-GMP with a different binding affinity. Intriguingly, a noncanonical PilZ domain has recently been found to serve as a mediator to link FimXEAL to the PilB or PilT ATPase to control the function of type IV pili (T4P). It is thus essential to determine the structure of the FimXEAL-PilZ complex in order to determine how the binding of c-di-GMP to the FimXEAL domain induces conformational change of the adjoining noncanonical PilZ domain, which may transmit information to PilB or PilT to control T4P function. Here, the preparation and preliminary X-ray diffraction studies of the XccFimXEAL-c-di-GMP and XccFimXEAL-c-di-GMP-XccPilZ complexes from Xcc (Xanthomonas campestris pv. campesteris) are reported. Detailed studies of these complexes may allow a more thorough understanding of how c-di-GMP transmits its effects through the degenerate EAL domain and the noncanonical PilZ domain.

บรรณานุกรม :
Liao, Yi-Ting , Chin, Ko-Hsin , Kuo, Wei-Ting , Chuah, Mary Lay-Cheng , Liang, Zhao-Xun , Chou, Shan-Ho . (2555). Crystallization and preliminary X-ray diffraction characterization of the XccFimXEAL-c-di-GMP and XccFimXEAL-c-di-GMP-XccPilZ complexes from Xanthomonas campestris.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Liao, Yi-Ting , Chin, Ko-Hsin , Kuo, Wei-Ting , Chuah, Mary Lay-Cheng , Liang, Zhao-Xun , Chou, Shan-Ho . 2555. "Crystallization and preliminary X-ray diffraction characterization of the XccFimXEAL-c-di-GMP and XccFimXEAL-c-di-GMP-XccPilZ complexes from Xanthomonas campestris".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Liao, Yi-Ting , Chin, Ko-Hsin , Kuo, Wei-Ting , Chuah, Mary Lay-Cheng , Liang, Zhao-Xun , Chou, Shan-Ho . "Crystallization and preliminary X-ray diffraction characterization of the XccFimXEAL-c-di-GMP and XccFimXEAL-c-di-GMP-XccPilZ complexes from Xanthomonas campestris."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Liao, Yi-Ting , Chin, Ko-Hsin , Kuo, Wei-Ting , Chuah, Mary Lay-Cheng , Liang, Zhao-Xun , Chou, Shan-Ho . Crystallization and preliminary X-ray diffraction characterization of the XccFimXEAL-c-di-GMP and XccFimXEAL-c-di-GMP-XccPilZ complexes from Xanthomonas campestris. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.