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Crystallization and preliminary X-ray crystallographic analysis of subunit F (F1-94), an essential coupling subunit of the eukaryotic V1VO-ATPase from Saccharomyces cerevisiae

หน่วยงาน Nanyang Technological University, Singapore

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ชื่อเรื่อง : Crystallization and preliminary X-ray crystallographic analysis of subunit F (F1-94), an essential coupling subunit of the eukaryotic V1VO-ATPase from Saccharomyces cerevisiae
นักวิจัย : Basak, Sandip , Balakrishna, Asha Manikkoth , Manimekalai, Malathy Sony Subramanian , Grüber, Gerhard
คำค้น : DRNTU::Science::Chemistry::Biochemistry , DRNTU::Science::Chemistry::Crystallography
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Basak, S., Balakrishna, A. M., Manimekalai, M. S. S., & Grüber, G. (2012). Crystallization and preliminary X-ray crystallographic analysis of subunit F (F1-94), an essential coupling subunit of the eukaryotic V1VO-ATPase from Saccharomyces cerevisiae. Acta Crystallographica Section F Structural Biology and Crystallization Communications, 68(9), 1055-1059. , 1744-3091 , http://hdl.handle.net/10220/9180 , http://dx.doi.org/10.1107/S1744309112032526
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Acta crystallographica section F structural biology and crystallization communications
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

V-ATPases are very complex multi-subunit enzymes which function as proton-pumping rotary nanomotors. The rotary and coupling subunit F (F1-94) was crystallized by the hanging-drop vapour-diffusion method. The native crystals diffracted to a resolution of 2.64 Å and belonged to space group C2221, with unit-cell parameters a = 47.21, b = 160.26, c = 102.49 Å. The selenomethionyl form of the F1-94 I69M mutant diffracted to a resolution of 2.3 Å and belonged to space group C2221, with unit-cell parameters a = 47.22, b = 160.83, c = 102.74 Å. Initial phasing and model building suggested the presence of four molecules in the asymmetric unit.

บรรณานุกรม :
Basak, Sandip , Balakrishna, Asha Manikkoth , Manimekalai, Malathy Sony Subramanian , Grüber, Gerhard . (2555). Crystallization and preliminary X-ray crystallographic analysis of subunit F (F1-94), an essential coupling subunit of the eukaryotic V1VO-ATPase from Saccharomyces cerevisiae.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Basak, Sandip , Balakrishna, Asha Manikkoth , Manimekalai, Malathy Sony Subramanian , Grüber, Gerhard . 2555. "Crystallization and preliminary X-ray crystallographic analysis of subunit F (F1-94), an essential coupling subunit of the eukaryotic V1VO-ATPase from Saccharomyces cerevisiae".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Basak, Sandip , Balakrishna, Asha Manikkoth , Manimekalai, Malathy Sony Subramanian , Grüber, Gerhard . "Crystallization and preliminary X-ray crystallographic analysis of subunit F (F1-94), an essential coupling subunit of the eukaryotic V1VO-ATPase from Saccharomyces cerevisiae."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Basak, Sandip , Balakrishna, Asha Manikkoth , Manimekalai, Malathy Sony Subramanian , Grüber, Gerhard . Crystallization and preliminary X-ray crystallographic analysis of subunit F (F1-94), an essential coupling subunit of the eukaryotic V1VO-ATPase from Saccharomyces cerevisiae. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.