ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Ribosome engineering to promote new crystal forms

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Ribosome engineering to promote new crystal forms
นักวิจัย : Selmer, Maria , Gao, Yong-Gui , Weixlbaumer, Albert , Ramakrishnan, V.
คำค้น : DRNTU::Science::Chemistry::Biochemistry , DRNTU::Science::Chemistry::Crystallography
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Selmer, M., Gao, Y.-G., Weixlbaumer, A., & Ramakrishnan, V. (2012). Ribosome engineering to promote new crystal forms. Acta Crystallographica Section D Biological Crystallography, 68(5), 578-583. , 0907-4449 , http://hdl.handle.net/10220/9266 , http://dx.doi.org/10.1107/S0907444912006348
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Acta crystallographica section D biological crystallography
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Crystallographic studies of the ribosome have provided molecular details of protein synthesis. However, the crystallization of functional complexes of ribosomes with GTPase translation factors proved to be elusive for a decade after the first ribosome structures were determined. Analysis of the packing in different 70S ribosome crystal forms revealed that regardless of the species or space group, a contact between ribosomal protein L9 from the large subunit and 16S rRNA in the shoulder of a neighbouring small subunit in the crystal lattice competes with the binding of GTPase elongation factors to this region of 16S rRNA. To prevent the formation of this preferred crystal contact, a mutant strain of Thermus thermophilus, HB8-MRCMSAW1, in which the ribosomal protein L9 gene has been truncated was constructed by homologous recombination. Mutant 70S ribosomes were used to crystallize and solve the structure of the ribosome with EF-G, GDP and fusidic acid in a previously unobserved crystal form. Subsequent work has shown the usefulness of this strain for crystallization of the ribosome with other GTPase factors.

บรรณานุกรม :
Selmer, Maria , Gao, Yong-Gui , Weixlbaumer, Albert , Ramakrishnan, V. . (2555). Ribosome engineering to promote new crystal forms.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Selmer, Maria , Gao, Yong-Gui , Weixlbaumer, Albert , Ramakrishnan, V. . 2555. "Ribosome engineering to promote new crystal forms".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Selmer, Maria , Gao, Yong-Gui , Weixlbaumer, Albert , Ramakrishnan, V. . "Ribosome engineering to promote new crystal forms."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Selmer, Maria , Gao, Yong-Gui , Weixlbaumer, Albert , Ramakrishnan, V. . Ribosome engineering to promote new crystal forms. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.