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Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor
นักวิจัย : Rai, Varun , Hapuarachchi, Hapuarachchige C. , Ng, Lee Ching , Soh, Siew Hwa , Leo, Yee Sin , Toh, Chee-Seng
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Rai, V., Hapuarachchi, H. C., Ng, L. C., Soh, S. H., Leo, Y. S., & Toh, C. S. (2012). Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor. PLoS ONE, 7(8). , 1932-6203 , http://hdl.handle.net/10220/9193 , http://dx.doi.org/10.1371/journal.pone.0042346
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : PLoS ONE
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

A nanoporous alumina membrane-based ultrasensitive DNA biosensor is constructed using 5′-aminated DNA probes immobilized onto the alumina channel walls. Alumina nanoporous membrane-like structure is carved over platinum wire electrode of 76 µm diameter dimension by electrochemical anodization. The hybridization of complementary target DNA with probe DNA molecules attached inside the pores influences the pore size and ionic conductivity. The biosensor demonstrates linear range over 6 order of magnitude with ultrasensitive detection limit of 9.55×10−12 M for the quantification of ss-31 mer DNA sequence. Its applicability is challenged against real time cDNA PCR sample of dengue virus serotype1 derived from asymmetric PCR. Excellent specificity down to one nucleotide mismatch in target DNA sample of DENV3 is also demonstrated.

บรรณานุกรม :
Rai, Varun , Hapuarachchi, Hapuarachchige C. , Ng, Lee Ching , Soh, Siew Hwa , Leo, Yee Sin , Toh, Chee-Seng . (2555). Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Rai, Varun , Hapuarachchi, Hapuarachchige C. , Ng, Lee Ching , Soh, Siew Hwa , Leo, Yee Sin , Toh, Chee-Seng . 2555. "Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Rai, Varun , Hapuarachchi, Hapuarachchige C. , Ng, Lee Ching , Soh, Siew Hwa , Leo, Yee Sin , Toh, Chee-Seng . "Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Rai, Varun , Hapuarachchi, Hapuarachchige C. , Ng, Lee Ching , Soh, Siew Hwa , Leo, Yee Sin , Toh, Chee-Seng . Ultrasensitive cDNA detection of dengue virus RNA using electrochemical nanoporous membrane-based biosensor. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.