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Hypoxia activated EGFR signaling induces epithelial to mesenchymal transition (EMT)

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Hypoxia activated EGFR signaling induces epithelial to mesenchymal transition (EMT)
นักวิจัย : Misra, Ashish , Pandey, Chhiti , Sze, Siu Kwan , Thanabalu, Thirumaran
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Misra, A., Pandey, C., Sze, S. K., & Thanabalu, T. (2012). Hypoxia activated EGFR signaling induces epithelial to mesenchymal transition (EMT). PLoS ONE, 7(11). , 1932-6203 , http://hdl.handle.net/10220/9207 , http://dx.doi.org/10.1371/journal.pone.0049766
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : PLoS ONE
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Metastasis is a multi-step process which requires the conversion of polarized epithelial cells to mesenchymal cells, Epithelial–Mesenchymal Transition (EMT). EMT is essential during embryonic morphogenesis and has been implicated in the progression of primary tumors towards metastasis. Hypoxia is known to induce EMT; however the molecular mechanism is still poorly understood. Using the A431 epithelial cancer cell line, we show that cells grown under hypoxic conditions migrated faster than cells grown under normal oxygen environment. Cells grown under hypoxia showed reduced adhesion to the extracellular matrix (ECM) probably due to reduced number of Vinculin patches. Growth under hypoxic conditions also led to down regulation of E-cadherin and up regulation of vimentin expression. The increased motility of cells grown under hypoxia could be due to redistribution of Rac1 to the plasma membrane as opposed to increased expression of Rac1. EGF (Epidermal Growth Factor) is a known inducer of EMT and growth of A431 cells in the absence of oxygen led to increased expression of EGFR (EGF Receptor). Treatment of A431 cells with EGF led to reduced cell adhesion to ECM, increased cell motility and other EMT characteristics. Furthermore, this transition was blocked by the monoclonal antibody Cetuximab. Cetuximab also blocked the hypoxia-induced EMT suggesting that cell growth under hypoxic conditions led to activation of EGFR signaling and induction of EMT phenotype.

บรรณานุกรม :
Misra, Ashish , Pandey, Chhiti , Sze, Siu Kwan , Thanabalu, Thirumaran . (2555). Hypoxia activated EGFR signaling induces epithelial to mesenchymal transition (EMT).
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Misra, Ashish , Pandey, Chhiti , Sze, Siu Kwan , Thanabalu, Thirumaran . 2555. "Hypoxia activated EGFR signaling induces epithelial to mesenchymal transition (EMT)".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Misra, Ashish , Pandey, Chhiti , Sze, Siu Kwan , Thanabalu, Thirumaran . "Hypoxia activated EGFR signaling induces epithelial to mesenchymal transition (EMT)."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Misra, Ashish , Pandey, Chhiti , Sze, Siu Kwan , Thanabalu, Thirumaran . Hypoxia activated EGFR signaling induces epithelial to mesenchymal transition (EMT). กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.