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Intrinsic differences between authentic and cryptic 5' splice sites

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Intrinsic differences between authentic and cryptic 5' splice sites
นักวิจัย : Roca, Xavier , Sachidanandam, Ravi , Krainer, Adrian R.
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2546
อ้างอิง : Roca, X., Sachidanandam R., & Krainer A. R. (2003). Intrinsic differences between authentic and cryptic 5' splice sites. Nucleic Acids Research, 31(21), 6321-6333. , 1362-4962 , http://hdl.handle.net/10220/8876 , http://dx.doi.org/10.1093/nar/gkg830
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Nucleic acids research
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Cryptic splice sites are used only when use of a natural splice site is disrupted by mutation. To determine the features that distinguish authentic from cryptic 5′ splice sites (5′ss), we systematically analyzed a set of 76 cryptic 5′ss derived from 46 human genes. These cryptic 5′ss have a similar frequency distribution in exons and introns, and are usually located close to the authentic 5′ss. Statistical analysis of the strengths of the 5′ss using the Shapiro and Senapathy matrix revealed that authentic 5′ss have significantly higher score values than cryptic 5′ss, which in turn have higher values than the mutant ones. β‐Globin provides an interesting exception to this rule, so we chose it for detailed experimental analysis in vitro. We found that the sequences of the β‐globin authentic and cryptic 5′ss, but not their surrounding context, determine the correct 5′ss choice, although their respective scores do not reflect this functional difference. Our analysis provides a statistical basis to explain the competitive advantage of authentic over cryptic 5′ss in most cases, and should facilitate the development of tools to reliably predict the effect of disease‐associated 5′ss‐disrupting mutations at the mRNA level.

บรรณานุกรม :
Roca, Xavier , Sachidanandam, Ravi , Krainer, Adrian R. . (2546). Intrinsic differences between authentic and cryptic 5' splice sites.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Roca, Xavier , Sachidanandam, Ravi , Krainer, Adrian R. . 2546. "Intrinsic differences between authentic and cryptic 5' splice sites".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Roca, Xavier , Sachidanandam, Ravi , Krainer, Adrian R. . "Intrinsic differences between authentic and cryptic 5' splice sites."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2546. Print.
Roca, Xavier , Sachidanandam, Ravi , Krainer, Adrian R. . Intrinsic differences between authentic and cryptic 5' splice sites. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2546.