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Comprehensive splice-site analysis using comparative genomics

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Comprehensive splice-site analysis using comparative genomics
นักวิจัย : Sheth, Nihar , Roca, Xavier , Hastings, Michelle L. , Roeder, Ted , Krainer, Adrian R. , Sachidanandam, Ravi
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2549
อ้างอิง : Sheth, N., Roca, X., Hastings, M. L., Roeder, T., Krainer, A. R., & Sachidanandam, R. (2006). Comprehensive splice-site analysis using comparative genomics. Nucleic Acids Research, 34(14), 3955-3967. , http://hdl.handle.net/10220/8868 , http://dx.doi.org/10.1093/nar/gkl556
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Nucleic acids research
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

We have collected over half a million splice sites from five species—Homo sapiens, Mus musculus, Drosophila melanogaster, Caenorhabditis elegans and Arabidopsis thaliana—and classified them into four subtypes: U2-type GT–AG and GC–AG and U12-type GT–AG and AT–AC. We have also found new examples of rare splice-site categories, such as U12-type introns without canonical borders, and U2-dependent AT–AC introns. The splice-site sequences and several tools to explore them are available on a public website (SpliceRack). For the U12-type introns, we find several features conserved across species, as well as a clustering of these introns on genes. Using the information content of the splice-site motifs, and the phylogenetic distance between them, we identify: (i) a higher degree of conservation in the exonic portion of the U2-type splice sites in more complex organisms; (ii) conservation of exonic nucleotides for U12-type splice sites; (iii) divergent evolution of C.elegans 3′ splice sites (3′ss) and (iv) distinct evolutionary histories of 5′ and 3′ss. Our study proves that the identification of broad patterns in naturally-occurring splice sites, through the analysis of genomic datasets, provides mechanistic and evolutionary insights into pre-mRNA splicing.

บรรณานุกรม :
Sheth, Nihar , Roca, Xavier , Hastings, Michelle L. , Roeder, Ted , Krainer, Adrian R. , Sachidanandam, Ravi . (2549). Comprehensive splice-site analysis using comparative genomics.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Sheth, Nihar , Roca, Xavier , Hastings, Michelle L. , Roeder, Ted , Krainer, Adrian R. , Sachidanandam, Ravi . 2549. "Comprehensive splice-site analysis using comparative genomics".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Sheth, Nihar , Roca, Xavier , Hastings, Michelle L. , Roeder, Ted , Krainer, Adrian R. , Sachidanandam, Ravi . "Comprehensive splice-site analysis using comparative genomics."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2549. Print.
Sheth, Nihar , Roca, Xavier , Hastings, Michelle L. , Roeder, Ted , Krainer, Adrian R. , Sachidanandam, Ravi . Comprehensive splice-site analysis using comparative genomics. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2549.