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Molecular characterization of the recombinant A-chain of a Type II ribosome-inactivating protein (RIP) from viscum album coloratum and structural basis on its ribosome-inactivating activity and the sugar-binding properties of the B-chain

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Molecular characterization of the recombinant A-chain of a Type II ribosome-inactivating protein (RIP) from viscum album coloratum and structural basis on its ribosome-inactivating activity and the sugar-binding properties of the B-chain
นักวิจัย : Ye, Wen-Hui , Nanga, Ravi Prakash Reddy , Kang, Cong Bao , Song, Joo-Hye , Song, Seong Kyu , Yoon, Ho Sup
คำค้น : DRNTU::Science::Biological sciences::Microbiology.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2549
อ้างอิง : Ye, W., Nanga, R. P. R., Kang, C. B., Song, J. H., Song, S. K., & Yoon, H. S. (2006). Molecular characterization of the recombinant A-chain of a Type II ribosome-inactivating protein (RIP) from viscum album coloratum and structural basis on its ribosome-inactivating activity and the sugar-binding properties of the B-chain. Journal of Biochemistry and Molecular Biology, 39(5), 560-570. , http://hdl.handle.net/10220/8824 , http://www.jbmb.or.kr/fulltext/jbmb/view.php?vol=39&page=560
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of biochemistry and molecular biology
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Mistletoe (Viscum album) lectins, which are classified as a type II ribosome-inactivating protein (RIP) due to their unique biological function and the potential medical and therapeutic application in cancer cells, receive a rising attention. The heterodimeric glycoproteins contain the Achain with catalytic activity and the B-chain with sugar binding properties. In recent years, studies involving the lectins from the white berry European mistletoe (Viscum album) and the yellow berry Korean mistletoe (Viscum album coloratum) have been described. However, the detailed mechanism in exerting unique cytotoxic effect on cancer cells still remains unclear. Here, we aim to understand and define the molecular basis and biological effects of the type II RIPs, through the studies of the recombinant Korean mistletoe lectin. To this end, we expressed, purified the recombinant Korean mistletoe lectin (rKML), and investigated its molecular characteristics in vitro, its cytotoxicity and ability to induce apoptotic cell death in cancer cells. To gain structural basis for its catalytic activity and sugar binding properties, we performed homology modeling studies based on the high degree of sequence identity and conserved secondary structure prediction between Korean and European, Himalayan mistletoe lectins, and Ricin.

บรรณานุกรม :
Ye, Wen-Hui , Nanga, Ravi Prakash Reddy , Kang, Cong Bao , Song, Joo-Hye , Song, Seong Kyu , Yoon, Ho Sup . (2549). Molecular characterization of the recombinant A-chain of a Type II ribosome-inactivating protein (RIP) from viscum album coloratum and structural basis on its ribosome-inactivating activity and the sugar-binding properties of the B-chain.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ye, Wen-Hui , Nanga, Ravi Prakash Reddy , Kang, Cong Bao , Song, Joo-Hye , Song, Seong Kyu , Yoon, Ho Sup . 2549. "Molecular characterization of the recombinant A-chain of a Type II ribosome-inactivating protein (RIP) from viscum album coloratum and structural basis on its ribosome-inactivating activity and the sugar-binding properties of the B-chain".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ye, Wen-Hui , Nanga, Ravi Prakash Reddy , Kang, Cong Bao , Song, Joo-Hye , Song, Seong Kyu , Yoon, Ho Sup . "Molecular characterization of the recombinant A-chain of a Type II ribosome-inactivating protein (RIP) from viscum album coloratum and structural basis on its ribosome-inactivating activity and the sugar-binding properties of the B-chain."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2549. Print.
Ye, Wen-Hui , Nanga, Ravi Prakash Reddy , Kang, Cong Bao , Song, Joo-Hye , Song, Seong Kyu , Yoon, Ho Sup . Molecular characterization of the recombinant A-chain of a Type II ribosome-inactivating protein (RIP) from viscum album coloratum and structural basis on its ribosome-inactivating activity and the sugar-binding properties of the B-chain. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2549.