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Immobilization of recombinant vault nanoparticles on solid substrates

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Immobilization of recombinant vault nanoparticles on solid substrates
นักวิจัย : Xia, Yun , Ramgopal, Yamini , Li, Hai , Shang, Lei , Srinivas, Parisa , Kickhoefer, Valerie A. , Rome, Leonard H. , Preiser, Peter Rainer , Boey, Freddy Yin Chiang , Zhang, Hua , Venkatraman, Subbu S.
คำค้น : DRNTU::Engineering::Materials.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2553
อ้างอิง : Xia, Y., Ramgopal, Y., Li, H., Shang, L., Srinivas, P., Kickhoefer, V. A., et al. (2010). Immobilization of recombinant vault nanoparticles on solid substrates. ACS Nano, 4(3), 1417-1424. , 1936-0851 , http://hdl.handle.net/10220/8608 , http://dx.doi.org/10.1021/nn901167s
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : ACS nano
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Native vaults are nanoscale particles found abundantly in the cytoplasm of most eukaryotic cells. They have a capsule-like structure with a thin shell surrounding a “hollow” interior compartment. Recombinant vault particles were found to self-assemble following expression of the major vault protein (MVP) in a baculovirus expression system, and these particles are virtually identical to native vaults. Such particles have been recently studied as potential delivery vehicles. In this study, we focus on immobilization of vault particles on a solid substrate, such as glass, as a first step to study their interactions with cells. To this end, we first engineered the recombinant vaults by fusing two different tags to the C-terminus of MVP, a 3 amino acid RGD peptide and a 12 amino acid RGD-strep-tag peptide. We have demonstrated two strategies for immobilizing vaults on solid substrates. The barrel-and-cap structure of vault particles was observed for the first time, by atomic force microscopy (AFM), in a dry condition. This work proved the feasibility of immobilizing vault nanoparticles on a material surface, and the possibility of using vault nanoparticles as localized and sustainable drug carriers as well as a biocompatible surface moiety.

บรรณานุกรม :
Xia, Yun , Ramgopal, Yamini , Li, Hai , Shang, Lei , Srinivas, Parisa , Kickhoefer, Valerie A. , Rome, Leonard H. , Preiser, Peter Rainer , Boey, Freddy Yin Chiang , Zhang, Hua , Venkatraman, Subbu S. . (2553). Immobilization of recombinant vault nanoparticles on solid substrates.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Xia, Yun , Ramgopal, Yamini , Li, Hai , Shang, Lei , Srinivas, Parisa , Kickhoefer, Valerie A. , Rome, Leonard H. , Preiser, Peter Rainer , Boey, Freddy Yin Chiang , Zhang, Hua , Venkatraman, Subbu S. . 2553. "Immobilization of recombinant vault nanoparticles on solid substrates".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Xia, Yun , Ramgopal, Yamini , Li, Hai , Shang, Lei , Srinivas, Parisa , Kickhoefer, Valerie A. , Rome, Leonard H. , Preiser, Peter Rainer , Boey, Freddy Yin Chiang , Zhang, Hua , Venkatraman, Subbu S. . "Immobilization of recombinant vault nanoparticles on solid substrates."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2553. Print.
Xia, Yun , Ramgopal, Yamini , Li, Hai , Shang, Lei , Srinivas, Parisa , Kickhoefer, Valerie A. , Rome, Leonard H. , Preiser, Peter Rainer , Boey, Freddy Yin Chiang , Zhang, Hua , Venkatraman, Subbu S. . Immobilization of recombinant vault nanoparticles on solid substrates. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2553.