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Efficient intracellular delivery of functional proteins using cationic polymer core/shell nanoparticles

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Efficient intracellular delivery of functional proteins using cationic polymer core/shell nanoparticles
นักวิจัย : Lee, Ashlynn L. Z. , Wang, Yong , Ye, Wen-Hui , Yoon, Ho Sup , Chan, Sui Yung , Yang, Yi-Yan
คำค้น : DRNTU::Engineering::Materials::Biomaterials.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2550
อ้างอิง : Lee, A. L., Wang, Y., Ye, W. H., Yoon, H. S., Chan, S. Y., & Yang, Y. Y. (2008). Efficient intracellular delivery of functional proteins using cationic polymer core/shell nanoparticles. Biomaterials, 29(9), 1224-1232. , 01429612 , http://hdl.handle.net/10220/8522 , http://dx.doi.org/10.1016/j.biomaterials.2007.11.021
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Biomaterials
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Cationic core/shell nanoparticles self-assembled from biodegradable, cationic and amphiphilic copolymer poly{N-methyldietheneaminesebacate)-co-[(cholesteryloxocarbonylamido ethyl) methyl bis(ethylene) ammonium bromide] sebacate}, P(MDS-co-CES), were fabricated and employed to deliver lectin A-chain, an anticancer glycoprotein. Lectin A-chain was efficiently bound onto the surfaces of the nanoparticles at high mass ratios of nanoparticles to lectin A-chain. The nanoparticle/lectin A-chain complexes had an average size of approximately 150 nm with zeta potential of about +30 mV at the mass ratio of 50 or above while the BioPorter/lectin A-chain complexes had a larger particle size and relatively lower zeta potential (150 nm vs. 455 nm; +30 mV vs. +20 mV). Therefore, the cellular uptake of nanoparticle/lectin A-chain com-plexes was much greater than that of BioPorter/lectin A-chain complexes. The results obtained from cytotoxicity tests show that lectin A-chain delivered by the nanoparticles was significantly more toxic against MDA-MB-231, HeLa, HepG2 and 4T1 cell lines when compared to Bio-Porter, and IC50 of lectin A-chain delivered by the nanoparticles was 0.2, 0.5, 10 and 50 mg/l, respectively, while that of lectin A-chain delivered by BioPorter was higher than 100 mg/l in all cell lines tested. These nano-sized particles may provide an efficient approach for intracellular delivery of biologically active proteins.

บรรณานุกรม :
Lee, Ashlynn L. Z. , Wang, Yong , Ye, Wen-Hui , Yoon, Ho Sup , Chan, Sui Yung , Yang, Yi-Yan . (2550). Efficient intracellular delivery of functional proteins using cationic polymer core/shell nanoparticles.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Lee, Ashlynn L. Z. , Wang, Yong , Ye, Wen-Hui , Yoon, Ho Sup , Chan, Sui Yung , Yang, Yi-Yan . 2550. "Efficient intracellular delivery of functional proteins using cationic polymer core/shell nanoparticles".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Lee, Ashlynn L. Z. , Wang, Yong , Ye, Wen-Hui , Yoon, Ho Sup , Chan, Sui Yung , Yang, Yi-Yan . "Efficient intracellular delivery of functional proteins using cationic polymer core/shell nanoparticles."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2550. Print.
Lee, Ashlynn L. Z. , Wang, Yong , Ye, Wen-Hui , Yoon, Ho Sup , Chan, Sui Yung , Yang, Yi-Yan . Efficient intracellular delivery of functional proteins using cationic polymer core/shell nanoparticles. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2550.