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Influence of histone tails and H4 tail acetylations on nucleosome–nucleosome interactions

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Influence of histone tails and H4 tail acetylations on nucleosome–nucleosome interactions
นักวิจัย : Liu, Ying , Lu, Chenning , Yang, Ye , Fan, Yanping , Yang, Renliang , Liu, Chuan Fa , Korolev, Nikolay , Nordenskiöld, Lars
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2554
อ้างอิง : Liu, Y., Lu, C., Yang, Y., Fan, Y., Yang, R., Liu, C. F., et al. (2011). Influence of histone tails and H4 tail acetylations on nucleosome–nucleosome interactions. Journal of molecular biology, 414(5), 749–764. , http://hdl.handle.net/10220/8142 , http://dx.doi.org/10.1016/j.jmb.2011.10.031 , 165585
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of molecular biology
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Nucleosome–nucleosome interaction plays a fundamental role in chromatin folding and self-association. The cation-induced condensation of nucleosome core particles (NCPs) displays properties similar to those of chromatin fibers, with important contributions from the N-terminal histone tails. We study the self-association induced by addition of cations [Mg2+, Ca2+, cobalt(III)hexammine3+, spermidine3+ and spermine4+] for NCPs reconstituted with wild-type unmodified histones and with globular tailless histones and for NCPs with the H4 histone tail having lysine (K) acetylations or lysine-to-glutamine mutations at positions K5, K8, K12 and K16. In addition, the histone construct with the single H4K16 acetylation was investigated. Acetylated histones were prepared by a semisynthetic native chemical ligation method. The aggregation behavior of NCPs shows a general cation-dependent behavior similar to that of the self-association of nucleosome arrays. Unlike nucleosome array self-association, NCP aggregation is sensitive to position and nature of the H4 tail modification. The tetra-acetylation in the H4 tail significantly weakens the nucleosome–nucleosome interaction, while the H4 K → Q tetra-mutation displays a more modest effect. The single H4K16 acetylation also weakens the self-association of NCPs, which reflects the specific role of H4K16 in the nucleosome–nucleosome stacking. Tailless NCPs can aggregate in the presence of oligocations, which indicates that attraction also occurs by tail-independent nucleosome–nucleosome stacking and DNA–DNA attraction in the presence of cations. The experimental data were compared with the results of coarse-grained computer modeling for NCP solutions with explicit presence of mobile ions.

บรรณานุกรม :
Liu, Ying , Lu, Chenning , Yang, Ye , Fan, Yanping , Yang, Renliang , Liu, Chuan Fa , Korolev, Nikolay , Nordenskiöld, Lars . (2554). Influence of histone tails and H4 tail acetylations on nucleosome–nucleosome interactions.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Liu, Ying , Lu, Chenning , Yang, Ye , Fan, Yanping , Yang, Renliang , Liu, Chuan Fa , Korolev, Nikolay , Nordenskiöld, Lars . 2554. "Influence of histone tails and H4 tail acetylations on nucleosome–nucleosome interactions".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Liu, Ying , Lu, Chenning , Yang, Ye , Fan, Yanping , Yang, Renliang , Liu, Chuan Fa , Korolev, Nikolay , Nordenskiöld, Lars . "Influence of histone tails and H4 tail acetylations on nucleosome–nucleosome interactions."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2554. Print.
Liu, Ying , Lu, Chenning , Yang, Ye , Fan, Yanping , Yang, Renliang , Liu, Chuan Fa , Korolev, Nikolay , Nordenskiöld, Lars . Influence of histone tails and H4 tail acetylations on nucleosome–nucleosome interactions. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2554.