ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Nested PCR in magnetically actuated circular closed-loop PCR microchip system

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Nested PCR in magnetically actuated circular closed-loop PCR microchip system
นักวิจัย : Lok, Khoi Seng , Lee, Peter Peng Foo , Kwok, Yien Chian , Nguyen, Nam-Trung
คำค้น : DRNTU::Engineering::Mechanical engineering.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Lok, K. S., Lee, P. P. F., Kwok, Y. C., & Nguyen, N. T. (2012). Nested PCR in magnetically actuated circular closed-loop PCR microchip system. Microchimica Acta , 177(1-2), 111-117. , http://hdl.handle.net/10220/7905 , http://dx.doi.org/10.1007/s00604-012-0760-2 , 163867
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Microchimica acta
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

We demonstrate a new and sensitive amplification technique (referred to as Nested Polymerase Chain Reaction; nPCR). It based on a magnetically actuated circular closed-loop PCR microchip system. nPCR involves the use of two sets of primers in two successive PCR runs, and allows the amplification of a single locus from a minute quantity of template DNA. Two sets of primers are specially designed to a target 500-bp region of the bacteriophage lambda template DNA in the first PCR run, and a 247-bp region of the targeted 500-bp first PCR product in the second PCR run. PCR is run on the microchip system and concurrently in regular thermocycler for comparison. The products are analyzed by conventional agarose gel electrophoresis. The detection limit for the initial template DNA is 1.63 × 105 copies per μL (or 8.67 pg) for the first PCR run, and 1.63 copies per μL (or 0.0867 fg) for the second run. The results are comparable to a regular thermocycler. This preliminary study opens a new gateway to future development of specialized nPCR on chip.

บรรณานุกรม :
Lok, Khoi Seng , Lee, Peter Peng Foo , Kwok, Yien Chian , Nguyen, Nam-Trung . (2555). Nested PCR in magnetically actuated circular closed-loop PCR microchip system.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Lok, Khoi Seng , Lee, Peter Peng Foo , Kwok, Yien Chian , Nguyen, Nam-Trung . 2555. "Nested PCR in magnetically actuated circular closed-loop PCR microchip system".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Lok, Khoi Seng , Lee, Peter Peng Foo , Kwok, Yien Chian , Nguyen, Nam-Trung . "Nested PCR in magnetically actuated circular closed-loop PCR microchip system."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Lok, Khoi Seng , Lee, Peter Peng Foo , Kwok, Yien Chian , Nguyen, Nam-Trung . Nested PCR in magnetically actuated circular closed-loop PCR microchip system. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.