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Roles of cholesterol in vesicle fusion and motion

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Roles of cholesterol in vesicle fusion and motion
นักวิจัย : Zhang, Jing , Xue, Renhao , Ong, Wei-Yi , Chen, Peng
คำค้น : DRNTU::Science::Medicine::Biomedical engineering.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2552
อ้างอิง : Zhang, J., Xue, R., Ong, W. Y., & Chen, P. (2009). Roles of cholesterol in vesicle fusion and motion. Biophysical Journal, 97(5), 1371–1380. , http://hdl.handle.net/10220/7527 , http://dx.doi.org/10.1016/j.bpj.2009.06.025 , 149064
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Biophysical journal
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Although it is well established that exocytosis of neurotransmitters and hormones is highly regulated by numerous secretory proteins, such as SNARE proteins, there is an increasing appreciation of the importance of the chemophysical properties and organization of membrane lipids to various aspects of the exocytotic program. Based on amperometric recordings by carbon fiber microelectrodes, we show that deprivation of membrane cholesterol by methyl-b-cyclodextrin not only inhibited the extent of membrane depolarization-induced exocytosis, it also adversely affected the kinetics and quantal size of vesicle fusion in neuroendocrine PC12 cells. In addition, total internal fluorescence microscopy studies revealed that cholesterol depletion impaired vesicle docking and trafficking, which are believed to correlate with the dynamics of exocytosis. Furthermore, we found that free cholesterol is able to directly trigger vesicle fusion, albeit with less potency and slower kinetics as compared to membrane depolarization stimulation. These results underscore the versatile roles of cholesterol in facilitating exocytosis.

บรรณานุกรม :
Zhang, Jing , Xue, Renhao , Ong, Wei-Yi , Chen, Peng . (2552). Roles of cholesterol in vesicle fusion and motion.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Zhang, Jing , Xue, Renhao , Ong, Wei-Yi , Chen, Peng . 2552. "Roles of cholesterol in vesicle fusion and motion".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Zhang, Jing , Xue, Renhao , Ong, Wei-Yi , Chen, Peng . "Roles of cholesterol in vesicle fusion and motion."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2552. Print.
Zhang, Jing , Xue, Renhao , Ong, Wei-Yi , Chen, Peng . Roles of cholesterol in vesicle fusion and motion. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2552.