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Investigating circular dorsal ruffles through varying substrate stiffness and mathematical modeling

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Investigating circular dorsal ruffles through varying substrate stiffness and mathematical modeling
นักวิจัย : Zeng, Yukai , Lai, Tanny , Koh, Cheng Gee , LeDuc, Philip R. , Chiam, Keng-Hwee
คำค้น : DRNTU::Science::Biological sciences::Biophysics
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2554
อ้างอิง : Zeng, Y., Lai, T., Koh, C. G., LeDuc, P. R., Chiam, K.-H. (2011). Investigating circular dorsal ruffles through varying substrate stiffness and mathematical modelling. Biophysical journal, 101(9), 2122-2130. , 1542-0086 , http://hdl.handle.net/10220/7375 , http://dx.doi.org/10.1016/j.bpj.2011.09.047 , 162375
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Biophysical journal
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Circular dorsal ruffles (CDRs) are transient actin-rich ring-like structures which form on the dorsal surface of growth-factor stimulated cells. However, the dynamics and mechanism of formation of CDRs are still unknown. It has been observed that CDR formation leads to stress fibers disappearing near the CDRs. Since stress fiber formation can be modified by substrate stiffness, we examined the effect of substrate stiffness on CDR formation by seeding NIH 3T3 fibroblasts on glass and polydimethylsiloxane (PDMS) substrates of varying stiffnesses from 20 kPa to 1800 kPa. We found that increasing substrate stiffness increased the lifetime of the CDRs. We developed a mathematical model of the signaling pathways involved in CDR formation to provide insight into this lifetime and size dependence which is linked to substrate stiffness via Rac-Rho antagonism. From the model, increasing stiffness raised mDia1-nucleated stress fiber formation due to Rho activation. The increased stress fibers present increased replenishment of the G-actin pool, therefore prolonging Arp2/3-nucleated CDR formation due to Rac activation. Negative feedback by WAVE-related RacGAP on Rac explained how CDR actin propagates as an excitable wave, much like wave propagation in other excitable medium, e.g., nerve signal transmission.

บรรณานุกรม :
Zeng, Yukai , Lai, Tanny , Koh, Cheng Gee , LeDuc, Philip R. , Chiam, Keng-Hwee . (2554). Investigating circular dorsal ruffles through varying substrate stiffness and mathematical modeling.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Zeng, Yukai , Lai, Tanny , Koh, Cheng Gee , LeDuc, Philip R. , Chiam, Keng-Hwee . 2554. "Investigating circular dorsal ruffles through varying substrate stiffness and mathematical modeling".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Zeng, Yukai , Lai, Tanny , Koh, Cheng Gee , LeDuc, Philip R. , Chiam, Keng-Hwee . "Investigating circular dorsal ruffles through varying substrate stiffness and mathematical modeling."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2554. Print.
Zeng, Yukai , Lai, Tanny , Koh, Cheng Gee , LeDuc, Philip R. , Chiam, Keng-Hwee . Investigating circular dorsal ruffles through varying substrate stiffness and mathematical modeling. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2554.