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In vitro studies of magnetically enhanced transfection in COS-7 cells

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : In vitro studies of magnetically enhanced transfection in COS-7 cells
นักวิจัย : Ang, Derrick , Tay, Chor Yong , Tan, L. P. , Preiser, Peter Rainer , Ramanujan, Raju V.
คำค้น : DRNTU::Engineering::Materials::Magnetic materials.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2554
อ้างอิง : Ang, D., Tay, C. Y., Tan, L. P., Preiser, P. R., & Ramanujan, R. V. (2011). In vitro studies of magnetically enhanced transfection in COS-7 cells. Materials science and engineering : C, 31, 1455-1457. , http://hdl.handle.net/10220/7267 , http://dx.doi.org/10.1016/j.msec.2011.05.014 , 161870
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Materials science and engineering : C
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

In the magnetically enhanced gene delivery technique, DNA complexed with polymer coated aggregated magnetic nanoparticles (AMNPs) is used for effecting transfection. The aim of this study is to examine the relationship between transfection efficiency and the physical characteristics of the polymer coated AMNPs. In vitro studies of transfection efficiency in COS-7 cells were carried out using pEGFP-N1 and pMIR-REPORT complexed polyethylenimine (PEI) coated iron oxide magnetic nanoparticles. PEI coated AMNPs (PEI-AMNPs) with average individual particle diameters in the range of 8 nm to 30 nm were studied and characterized by transmission electron microscopy, vibrating sample magnetometry, X-ray diffractometry, thermal gravimetric analysis and photon correlation spectroscopy methods. PEI-A8MNP and PEI-A30MNP yielded higher transfection efficiency compared to commercial polyMAG particles as well as PEI of equivalent molar ratio of nitrogen/phosphorous (N/P ratio). The transfection efficiency was related to the physical characteristics of the PEI-AMNPs and its complexes: transfection efficiency was strongly positively correlated with saturation magnetization (Ms) and susceptibility (χ), strongly negatively correlated with N/P ratio, moderately positively correlated to zeta potential and moderately negatively correlated to hydrodynamic diameter of the complex. PEI-A8MNP and PEI-A30MNP possessing higher Ms, χ, lower N/P ratio and smaller complex size exhibited higher transfection efficiency compared to PEI-A16MNP which have weaker magnetic properties, higher N/P ratio and larger complex size.

บรรณานุกรม :
Ang, Derrick , Tay, Chor Yong , Tan, L. P. , Preiser, Peter Rainer , Ramanujan, Raju V. . (2554). In vitro studies of magnetically enhanced transfection in COS-7 cells.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ang, Derrick , Tay, Chor Yong , Tan, L. P. , Preiser, Peter Rainer , Ramanujan, Raju V. . 2554. "In vitro studies of magnetically enhanced transfection in COS-7 cells".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ang, Derrick , Tay, Chor Yong , Tan, L. P. , Preiser, Peter Rainer , Ramanujan, Raju V. . "In vitro studies of magnetically enhanced transfection in COS-7 cells."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2554. Print.
Ang, Derrick , Tay, Chor Yong , Tan, L. P. , Preiser, Peter Rainer , Ramanujan, Raju V. . In vitro studies of magnetically enhanced transfection in COS-7 cells. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2554.