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A hydrogel-based enzyme-loaded polymersome reactor

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : A hydrogel-based enzyme-loaded polymersome reactor
นักวิจัย : De Hoog, Hans-Peter M. , Arends, Isabel W. C. E. , Rowan, Alan E. , Cornelissen, Jeroen J. L. M. , Nolte, Roeland J. M.
คำค้น : DRNTU::Engineering::Materials::Biomaterials
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2553
อ้างอิง : De Hoog, H. P. M., Arends, I. W. C. E., Rowan, A. E., Cornelissen, J. J. L. M., & Nolte, R. J. M. (2010). A hydrogel-based enzyme-loaded polymersome reactor. Nanoscale, 2, 709-716. , 2040-3364 , http://hdl.handle.net/10220/6970 , http://dx.doi.org/10.1039/b9nr00325h , 155296
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Nanoscale
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

In this study we report the immobilization of enzyme-containing polymersomes into a macromolecular hydrogel. Whereas free enzyme shows progressive leakage from the hydrogel in a period of days, leakage of the polymersome-protected enzyme is virtually absent. The preparation of the hydrogel occurs under mild conditions and does not inhibit the activity of the encapsulated enzymes nor does it affect the structure of the polymersomes. The stability of the polymersome hydrogel architecture is demonstrated by the facile recycling of the polymersomes and their use in repeated reaction cycles. A ‘continuous-flow polymersome reactor’ is constructed in which substrate is added to the top of the reactor and product is collected at the bottom. This set-up allows the use of different enzymes and the processing of multiple substrates, as is demonstrated by the conversion of 2-methoxyphenyl acetate to tetraguaiacol in a reactor loaded with polymersome hydrogels containing the enzymes Candida antarcticalipase B (CALB) and glucose oxidase (GOx).

บรรณานุกรม :
De Hoog, Hans-Peter M. , Arends, Isabel W. C. E. , Rowan, Alan E. , Cornelissen, Jeroen J. L. M. , Nolte, Roeland J. M. . (2553). A hydrogel-based enzyme-loaded polymersome reactor.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
De Hoog, Hans-Peter M. , Arends, Isabel W. C. E. , Rowan, Alan E. , Cornelissen, Jeroen J. L. M. , Nolte, Roeland J. M. . 2553. "A hydrogel-based enzyme-loaded polymersome reactor".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
De Hoog, Hans-Peter M. , Arends, Isabel W. C. E. , Rowan, Alan E. , Cornelissen, Jeroen J. L. M. , Nolte, Roeland J. M. . "A hydrogel-based enzyme-loaded polymersome reactor."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2553. Print.
De Hoog, Hans-Peter M. , Arends, Isabel W. C. E. , Rowan, Alan E. , Cornelissen, Jeroen J. L. M. , Nolte, Roeland J. M. . A hydrogel-based enzyme-loaded polymersome reactor. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2553.