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Recognition of atypical 5' splice sites by shifted base-pairing to U1 snRNA

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Recognition of atypical 5' splice sites by shifted base-pairing to U1 snRNA
นักวิจัย : Roca, Xavier , Krainer, Adrian R.
คำค้น : DRNTU::Science::Biological sciences::Molecular biology.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2552
อ้างอิง : Roca, X., & Krainer, A. R. (2009). Recognition of atypical 5' splice sites by shifted base-pairing to U1 snRNA. Nature Structural and Molecular Biology, 16, 176-182. , http://hdl.handle.net/10220/6872 , http://dx.doi.org/10.1038/nsmb.1546
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Nature structural and molecular Biology
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Accurate pre-mRNA splicing is critical for gene expression. The 5' splice site (5' ss) — the highly diverse element at the 5' end of introns — is initially recognized via base-pairing to the 5' end of U1 small nuclear RNA (snRNA). However, many natural 5' ss have a very poor match to the consensus sequence, and are predicted to be very weak. Using genetic suppression experiments in human cells, we demonstrate that some atypical 5' ss are actually efficiently recognized by U1, in an alternative base-pairing register that is shifted by one nucleotide. These atypical 5' ss are phylogenetically widespread, and many of them are conserved. Moreover, shifted base-pairing provides an explanation for the effect of a 5' ss mutation associated with pontocerebellar hypoplasia. The unexpected flexibility in 5' ss/U1 base-pairing challenges an established paradigm, and has broad implications for splice-site prediction algorithms and gene-annotation efforts in genome projects.

บรรณานุกรม :
Roca, Xavier , Krainer, Adrian R. . (2552). Recognition of atypical 5' splice sites by shifted base-pairing to U1 snRNA.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Roca, Xavier , Krainer, Adrian R. . 2552. "Recognition of atypical 5' splice sites by shifted base-pairing to U1 snRNA".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Roca, Xavier , Krainer, Adrian R. . "Recognition of atypical 5' splice sites by shifted base-pairing to U1 snRNA."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2552. Print.
Roca, Xavier , Krainer, Adrian R. . Recognition of atypical 5' splice sites by shifted base-pairing to U1 snRNA. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2552.