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CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex
นักวิจัย : Choi, Yuk Kwan , Liu, Pengfei , Sze, Siu Kwan , Dai, Chao , Qi, Robert Z.
คำค้น : DRNTU::Science::Biological sciences::Molecular biology.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2553
อ้างอิง : Choi, Y. K., Liu, P., Sze, S. K., Dai, C., & Qi, R. Z. (2010). CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex. Journal of cell biology, 191(6), 1089-1095. , http://hdl.handle.net/10220/6796 , http://dx.doi.org/10.1083/jcb.201007030
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of cell biology
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

CDK5RAP2 is a human microcephaly protein that contains a γ-tubulin complex (γ-TuC)–binding domain conserved in Drosophila melanogaster centrosomin and Schizosaccharomyces pombe Mto1p and Pcp1p, which are γ-TuC–tethering proteins. In this study, we show that this domain within CDK5RAP2 associates with the γ-tubulin ring complex (γ-TuRC) to stimulate its microtubule-nucleating activity and is therefore referred to as the γ-TuRC–mediated nucleation activator (γ-TuNA). γ-TuNA but not its γ-TuC–binding-deficient mutant stimulates microtubule nucleation by purified γ-TuRC in vitro and induces extensive, γ-TuRC-dependent nucleation of microtubules in a microtubule regrowth assay. γ-TuRC bound to γ-TuNA contains NME7, FAM128A/B, and actin in addition to γ-tubulin and GCP2–6. RNA interference–mediated depletion of CDK5RAP2 impairs both centrosomal and acentrosomal microtubule nucleation, although γ-TuRC assembly is unaffected. Collectively, these results suggest that the γ-TuNA found in CDK5RAP2 has regulatory functions in γ-TuRC–mediated microtubule nucleation.

บรรณานุกรม :
Choi, Yuk Kwan , Liu, Pengfei , Sze, Siu Kwan , Dai, Chao , Qi, Robert Z. . (2553). CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Choi, Yuk Kwan , Liu, Pengfei , Sze, Siu Kwan , Dai, Chao , Qi, Robert Z. . 2553. "CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Choi, Yuk Kwan , Liu, Pengfei , Sze, Siu Kwan , Dai, Chao , Qi, Robert Z. . "CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2553. Print.
Choi, Yuk Kwan , Liu, Pengfei , Sze, Siu Kwan , Dai, Chao , Qi, Robert Z. . CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2553.