ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells
นักวิจัย : Kang, Tae Bong , Yoo, Yung Choon , Lee, Kwan Hee , Yoon, Ho Sup , Her, Erk , Kim, Jong Bae , Song, Seong Kyu
คำค้น : DRNTU::Science::Biological sciences::Microbiology::Immunology.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2551
อ้างอิง : Kang, T. B., Yoo, Y. C., Lee, K. H., Yoon, H. S., Her, E., Kim, J. B., et al. (2008). Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide(NO) production in murine macrophage cells. Journal of biomedical science, 15(2). , http://hdl.handle.net/10220/6780 , http://dx.doi.org/10.1007/s11373-007-9210-2
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of biomedical science
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Synthesis of nitric oxide (NO) is one of the important effector functions of innate immune cells. Although several reports have indicated mistletoe lectins induce immune cells to produce cytokines, studies regarding the activities of the lectins in the production of NO have been very limited. Here, we report on the induction of NO synthesis in a murine macrophage cell line, RAW264.7, by Korean mistletoe lectin (KML-IIU). When the macrophage cells were treated with KML-IIU in the presence of a suboptimal concentration of IFN-γ, NO production was induced in a concentration-dependent manner. Significantly higher levels of NO were induced by subchains of the KML-IIU (A and B), which have lower toxicities, as compared to the hololectin. Furthermore, expression of the inducible nitric oxide synthase (iNOS) gene was elevated in accordance with the level of NO production. When the synthase was inhibited by iNOS inhibitors (L-NIL and L-NAME), NO production was specifically reduced in a concentration-dependent manner. Our studies demonstrate that the KML-IIU and its subchains induce NO production in murine macrophage cells via activation of the iNOS gene expression, suggesting that the KML-IIU subchains may be used as an immunomodulator to enhance the effector functions of innate immune cells.

บรรณานุกรม :
Kang, Tae Bong , Yoo, Yung Choon , Lee, Kwan Hee , Yoon, Ho Sup , Her, Erk , Kim, Jong Bae , Song, Seong Kyu . (2551). Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Kang, Tae Bong , Yoo, Yung Choon , Lee, Kwan Hee , Yoon, Ho Sup , Her, Erk , Kim, Jong Bae , Song, Seong Kyu . 2551. "Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Kang, Tae Bong , Yoo, Yung Choon , Lee, Kwan Hee , Yoon, Ho Sup , Her, Erk , Kim, Jong Bae , Song, Seong Kyu . "Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2551. Print.
Kang, Tae Bong , Yoo, Yung Choon , Lee, Kwan Hee , Yoon, Ho Sup , Her, Erk , Kim, Jong Bae , Song, Seong Kyu . Korean mistletoe lectin (KML-IIU) and its subchains induce nitric oxide (NO) production in murine macrophage cells. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2551.