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Sorting catalytically active polymersome nanoreactors by flow cytometry

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Sorting catalytically active polymersome nanoreactors by flow cytometry
นักวิจัย : Nallani, Madhavan , Woestenenk, Rob , Hans-Peter M. de Hoog , Dongen, Stijn F. M. van , Boezeman, Jan , Cornelissen, Jeroen J. L. M. , Nolte, Roeland J. M. , Hest, Jan C. M. van.
คำค้น : DRNTU::Engineering::Materials::Biomaterials.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2551
อ้างอิง : Nallani, M., Woestenenk, R., Hoog, H. P. M. D., Dongen, S. F. M. V., Boezeman, J., Cornelissen, J. J. L. M., et al. (2009). Sorting Catalytically Active Polymersome Nanoreactors by Flow Cytometry. Small, 5(10), 1138-1143. , 1613-6810 , http://hdl.handle.net/10220/7282 , http://dx.doi.org/10.1002/smll.200801204 , 155293
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Small
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Flow cytometry is a powerful technique for high-throughput, fluorescence-activated screening and sorting of cells (FACS). This methodology has been extended by Griffiths to the screening of water-in-oil microdroplets filled with an in vitro protein expression system.[1–6] The catalytic gene product was detected by the transformation of a co-encapsulated profluorescent substrate into a fluorescent product. Here we report a strategy that involves a versatile one-step preparation procedure of enzyme filled porous and stable polymeric capsules (polymersomes). Since the pores of the capsules are small enough to keep enzymes in, whereas these are sufficiently large to allow (profluorescent) substrates to enter, enzyme activity screening can be performed by the build-up of fluorescence, followed by FACS. To prevent the substrate from diffusing out of the capsules, a trapping agent was added inside the capsule. With this technology we were able to separate enzymatically active polymersomes from non-filled or non-active polymersomes.

บรรณานุกรม :
Nallani, Madhavan , Woestenenk, Rob , Hans-Peter M. de Hoog , Dongen, Stijn F. M. van , Boezeman, Jan , Cornelissen, Jeroen J. L. M. , Nolte, Roeland J. M. , Hest, Jan C. M. van. . (2551). Sorting catalytically active polymersome nanoreactors by flow cytometry.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Nallani, Madhavan , Woestenenk, Rob , Hans-Peter M. de Hoog , Dongen, Stijn F. M. van , Boezeman, Jan , Cornelissen, Jeroen J. L. M. , Nolte, Roeland J. M. , Hest, Jan C. M. van. . 2551. "Sorting catalytically active polymersome nanoreactors by flow cytometry".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Nallani, Madhavan , Woestenenk, Rob , Hans-Peter M. de Hoog , Dongen, Stijn F. M. van , Boezeman, Jan , Cornelissen, Jeroen J. L. M. , Nolte, Roeland J. M. , Hest, Jan C. M. van. . "Sorting catalytically active polymersome nanoreactors by flow cytometry."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2551. Print.
Nallani, Madhavan , Woestenenk, Rob , Hans-Peter M. de Hoog , Dongen, Stijn F. M. van , Boezeman, Jan , Cornelissen, Jeroen J. L. M. , Nolte, Roeland J. M. , Hest, Jan C. M. van. . Sorting catalytically active polymersome nanoreactors by flow cytometry. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2551.