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Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors
นักวิจัย : Madhavan Nallani , Hans-Peter M. de Hoog , Jeroen J. L. M. Cornelissen , Alan E. Rowan , Roeland J. M. Nolte , Isabel W. C. E. Arends
คำค้น : DRNTU::Engineering::Materials::Biomaterials
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2552
อ้างอิง : Madhavan, N., Hans-Peter, M. H., Jeroen, J. L. M. C., Alan, E. R., Roeland, J. M. N., & Isabel, W. C. E. A. (2009). Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors. Organic and biomolecular chemistry, 7, 4604-4610. , 1477-0520 , http://hdl.handle.net/10220/6788 , http://dx.doi.org/10.1039/b911370c , 155295
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Organic and biomolecular chemistry
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The encapsulation of chloroperoxidase from Caldariomyces fumago (CPO) in block copolymer polymersomes is reported. Fluorescence and electron microscopy show that when the encapsulating conditions favour self-assembly of the block copolymer, the enzyme is incorporated with concentrations that are 50 times higher than the enzyme concentration before encapsulation. The oxidation of two substrates by the encapsulated enzyme was studied: i) pyrogallol, a common substrate used to assay CPO enzymatic activity and ii) thioanisole, of which the product, (R)-methyl phenyl sulfoxide, is an important pharmaceutical intermediate. The CPO-loaded polymersomes showed distinct reactivity towards these substrates.While the oxidation of pyrogallol was limited by diffusion of the substrate into the polymersome, the rate-limiting step for the oxidation of thioansiole was the turnover by the enzyme.

บรรณานุกรม :
Madhavan Nallani , Hans-Peter M. de Hoog , Jeroen J. L. M. Cornelissen , Alan E. Rowan , Roeland J. M. Nolte , Isabel W. C. E. Arends . (2552). Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Madhavan Nallani , Hans-Peter M. de Hoog , Jeroen J. L. M. Cornelissen , Alan E. Rowan , Roeland J. M. Nolte , Isabel W. C. E. Arends . 2552. "Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Madhavan Nallani , Hans-Peter M. de Hoog , Jeroen J. L. M. Cornelissen , Alan E. Rowan , Roeland J. M. Nolte , Isabel W. C. E. Arends . "Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2552. Print.
Madhavan Nallani , Hans-Peter M. de Hoog , Jeroen J. L. M. Cornelissen , Alan E. Rowan , Roeland J. M. Nolte , Isabel W. C. E. Arends . Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2552.