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A Phylogenetic Study of ~iPaecilomyces~i and Related Genera

หน่วยงาน ฐานข้อมูลวิทยานิพนธ์ไทย

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ชื่อเรื่อง : A Phylogenetic Study of ~iPaecilomyces~i and Related Genera
นักวิจัย : Janet Jennifer Luangsa-ard
คำค้น : -
หน่วยงาน : ฐานข้อมูลวิทยานิพนธ์ไทย
ผู้ร่วมงาน : -
ปีพิมพ์ : 2546
อ้างอิง : http://www.thaithesis.org/detail.php?id=1162546000039
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The genus Paecilomyces according to Samson (1974) is composed of twosections: Section Paecilomyces, including the type P. variotii, containingmesophilic, thermotolerant and thermophilic species mostly isolated from soiland food products and Section Isarioidea, containing mesophilic, mostly insectpathogenic species. The objective of this study was to evaluate the taxonomicvalue of DNA sequence data in relation to morphological characters, host range,ecology and anamorphs in order to determine the relationship between speciesdescribed by Samson (1974). Dr. R.A. Samson supplied strains including the typesof both sections and related Genera from the Centraalbureau voorSchimmelcultures. Entomogenous strains from Dr. Nigel Hywel-Jones were alsoused. Thermoresistant and thermotolerant strains of Section ~iPaecilomyces~iwere isolated from Thai soils at various locations using standard dilution platemethod. The 18S and ITS rDNA regions as well as the (3-tubulin gene were used toexamine the phylogenetic relationships of ~iPaecilomyces~i sensu lato.Phylogenetic analysis of the 18S rDNA demonstrates that Paecilomyces ispolyphyletic across two subclasses Sordariomycetidae and Eurotiomycetidae. Thetype species - ~iPaecilomyces variotii~iand thermophilic relatives belong in theorder Eurotiales (family Trichocomaceae) while mesophilic species related to~iPaecilomyces farinosus~i are in the order Hypocreales (familiesClavicipitaceae and Hypocreaceae). One species, ~iPaecilomyces inflatus~i, hadaffinities to the order Sordariales. Analyses of the two Sections using ITS and(3-tubulin gene show that within the Eurotiales Paecilomyces is polyphyletic andteleomorph connections are in need of clarification. There is a monophyleticgroup comprising of P. variotii, its teleomorph Talaromyces spectabilis and theByssochlamys species B. nivea, B. fulva and B. zollerniae. Five genotypes can bedistinguished in the (3-tubulin gene analysis of P. variotii isolates showing itis an aggregate of closely related taxa. Within the Hypocreales Paecilomyces ispolyphyletic although the taxa sampled failed to fully resolve relationships.There is a consistent clade in all analyses that is designated as the ~iIsaria~iclade comprising of ~iP. amoeneroseus, P. cateniannulatus, P. cateniobliquus,P. coleopterorum, P. farinosus, P. fumosoroseus, P. ghanensis, P. javanicus~iand ~iP. tenuipes~i. Molecular analysis has also verified the link between~iP.cinnamomeus~i and its teleomorph Torrubiella luteorostrata. Growth and temperature experiments show that isolates of the type strain~iP. variotii~i belonging to Section ~iPaecilomyces~i show differences in colonycolour and morphology when grown at 15 and 40(+,ฐ)C. In vitro synnemataldevelopment was investigated for ~iP.farinosus, P. javanicus~i and~iP.tenuipes~i on three kinds of media. Only ~iP.tenuipes~i produced synnematain culture. Formation of synnemata was found on Sabouraud Dextrose Yeast Agarafter two weeks of incubation at 25(+,ฐ)C. Sporulation started early on 2% MaltExtract Agar and mostly mycelial growth was found on Potato Dextrose Agar. Invivo and in vitro morphology proved to be highly plastic. Correlating morphologywith molecular phylogenies was problematic and needs further research.

บรรณานุกรม :
Janet Jennifer Luangsa-ard . (2546). A Phylogenetic Study of ~iPaecilomyces~i and Related Genera.
    กรุงเทพมหานคร : ฐานข้อมูลวิทยานิพนธ์ไทย.
Janet Jennifer Luangsa-ard . 2546. "A Phylogenetic Study of ~iPaecilomyces~i and Related Genera".
    กรุงเทพมหานคร : ฐานข้อมูลวิทยานิพนธ์ไทย.
Janet Jennifer Luangsa-ard . "A Phylogenetic Study of ~iPaecilomyces~i and Related Genera."
    กรุงเทพมหานคร : ฐานข้อมูลวิทยานิพนธ์ไทย, 2546. Print.
Janet Jennifer Luangsa-ard . A Phylogenetic Study of ~iPaecilomyces~i and Related Genera. กรุงเทพมหานคร : ฐานข้อมูลวิทยานิพนธ์ไทย; 2546.