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Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer

หน่วยงาน Universiti Sains Malaysia, Malaysia

รายละเอียด

ชื่อเรื่อง : Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer
นักวิจัย : Omar, Noorsharmimi , Loh, Qiuting , Gee, Jun Tye , Yee, Siew Choong , Noordin, Rahmah , Jörn, Glökler , Lim, Theam Soon
คำค้น : R735-854 Medical education. Medical schools. Research
หน่วยงาน : Universiti Sains Malaysia, Malaysia
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : http://eprints.usm.my/38675/1/Development_of_an_Antigen%2DDNAzyme_Based_Probe_for_a_Direct_Antibody%2DAntigen.pdf , Omar, Noorsharmimi and Loh, Qiuting and Gee, Jun Tye and Yee, Siew Choong and Noordin, Rahmah and Jörn, Glökler and Lim, Theam Soon (2014) Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer. Sensors, 14 (1). pp. 346-355. ISSN 1424-8220
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : https://doi.org/10.3390/s140100346 , http://eprints.usm.my/38675/
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the application of a G-rich daunomycin-specific aptamer for the development of an antibody-antigen detection assay. We investigated the ability of the daunomycin aptamer to efficiently catalyze the hemin-dependent peroxidase activity independent of daunomycin. A reporter probe consisting of biotinylated antigen and daunomycin aptamer coupled to streptavidin gold nanoparticles was successfully used to generate a colorimetric readout. In conclusion, the daunomycin aptamer can function as a robust alternative DNAzyme for the development of colorimetric assays.

บรรณานุกรม :
Omar, Noorsharmimi , Loh, Qiuting , Gee, Jun Tye , Yee, Siew Choong , Noordin, Rahmah , Jörn, Glökler , Lim, Theam Soon . (2557). Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer.
    กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia.
Omar, Noorsharmimi , Loh, Qiuting , Gee, Jun Tye , Yee, Siew Choong , Noordin, Rahmah , Jörn, Glökler , Lim, Theam Soon . 2557. "Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer".
    กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia.
Omar, Noorsharmimi , Loh, Qiuting , Gee, Jun Tye , Yee, Siew Choong , Noordin, Rahmah , Jörn, Glökler , Lim, Theam Soon . "Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer."
    กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia, 2557. Print.
Omar, Noorsharmimi , Loh, Qiuting , Gee, Jun Tye , Yee, Siew Choong , Noordin, Rahmah , Jörn, Glökler , Lim, Theam Soon . Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer. กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia; 2557.